Genomic Heterogeneity in Klebsiella pneumoniae Strains Is Associated with Primary Pyogenic Liver Abscess and Metastatic Infection
BackgroundPrimary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease Methods and resultsUsing DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake sy...
Gespeichert in:
| Veröffentlicht in: | The Journal of infectious diseases 2005-07, Vol.192 (1), p.117-128 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 128 |
|---|---|
| container_issue | 1 |
| container_start_page | 117 |
| container_title | The Journal of infectious diseases |
| container_volume | 192 |
| creator | Ma, Li-Chen Fang, Chi-Tai Lee, Cha-Ze Shun, Chia-Tung Wang, Jin-Town |
| description | BackgroundPrimary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease Methods and resultsUsing DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake system (kfu) a phosphoenolpyruvate sugar phosphotransferase system (PTS), and 6 unknown ORFs. The region was more prevalent among tissue-invasive strains (35/46) than among noninvasive strains (19/98) (P |
| doi_str_mv | 10.1086/430619 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_67913970</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30087094</jstor_id><oup_id>10.1086/430619</oup_id><sourcerecordid>30087094</sourcerecordid><originalsourceid>FETCH-LOGICAL-c451t-91fdce7c566fb810af7427a71a59e7d3424b89a9823e9f3336201c63542c3ab13</originalsourceid><addsrcrecordid>eNqFkV2LEzEUhgdR3O6q_0CJgt6N5muSyWUt67bYxUVXEW-GTHpG050mNcmovfSfmzJlC4J4lYv34Tk55y2KRwS_JLgWrzjDgqg7xYRUTJZCEHa3mGBMaUlqpU6K0xjXGGPOhLxfnJBKcaowmRS_L8D5jTVoDgmC_woObNoh69DbHtpooe812joYNt5ZDehDCtq6iBYRTWP0xuoEK_TTpm_oKtiNDjt0tdtrsnJpf0BA0zYaiBFpt0KXkHRMOuVw4TowyXr3oLjX6T7Cw8N7Vnx8c349m5fLdxeL2XRZGl6RVCrSrQxIUwnRtTXBupOcSi2JrhTIFeOUt7XSqqYMVMcYExQTI1jFqWG6JeyseDF6t8F_HyCmZmPzz_J-DvwQGyEVYUri_4JE1qKueJ3BZ3-Baz8El5doKGUKc4nro80EH2OArtmOd2oIbvbVNWN1GXxysA3tBlZH7NBVBp4fAB2N7rugnbHxyMncr6B70dOR88P238Mej8w6Jh9uKYZxLbHiOS_H3MYEv25zHW7ynZismvnnL8179uly9lrNmmv2B0YiwqY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>223904708</pqid></control><display><type>article</type><title>Genomic Heterogeneity in Klebsiella pneumoniae Strains Is Associated with Primary Pyogenic Liver Abscess and Metastatic Infection</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Ma, Li-Chen ; Fang, Chi-Tai ; Lee, Cha-Ze ; Shun, Chia-Tung ; Wang, Jin-Town</creator><creatorcontrib>Ma, Li-Chen ; Fang, Chi-Tai ; Lee, Cha-Ze ; Shun, Chia-Tung ; Wang, Jin-Town</creatorcontrib><description>BackgroundPrimary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease Methods and resultsUsing DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake system (kfu) a phosphoenolpyruvate sugar phosphotransferase system (PTS), and 6 unknown ORFs. The region was more prevalent among tissue-invasive strains (35/46) than among noninvasive strains (19/98) (P<.0001, χ2 test). To test the role played by this region in pathogenesis, 3 different deletion mutants (NTUH-K2044 [Δkfu], K2044 [ΔORF7–9], and K2044 [ΔPTS]) were constructed. Only the ΔkfuABC mutants showed decreased virulence in mice, compared with the wild-type strain. An in vitro assay confirmed the involvement of kfu in iron acquisition. There was a high correlation rate (85%) between the kfu/PTS region and 2 tissue invasion–associated chromosomal regions (allS and magA). Moreover, all 3 regions were present in strains that caused PLA plus endophthalmitis or meningitis ConclusionOur results suggest that chromosomal heterogeneity is present in tissue-invasive K. pneumoniae strains. A genotype containing all 3 regions is strongly associated with PLA and metastatic infection. These regions may serve as convenient markers for the rapid diagnosis of emergent tissue-invasive strains</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/430619</identifier><identifier>PMID: 15942901</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animals ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Chromosome Mapping ; Endophthalmitis ; Endophthalmitis - microbiology ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genome, Bacterial ; Genomes ; Genomics ; Humans ; Infections ; Infectious diseases ; Klebsiella Infections - microbiology ; Klebsiella pneumoniae ; Klebsiella pneumoniae - genetics ; Klebsiella pneumoniae - pathogenicity ; Liver abscess ; Liver Abscess, Pyogenic - microbiology ; Medical sciences ; Meningitis, Bacterial - microbiology ; Mice ; Mice, Inbred BALB C ; Microarray Analysis ; Microbiology ; Miscellaneous ; Open reading frames ; Operator regions ; Plasmids ; Polymerase chain reaction ; Sepsis - microbiology ; Tumors ; Virulence</subject><ispartof>The Journal of infectious diseases, 2005-07, Vol.192 (1), p.117-128</ispartof><rights>Copyright 2005 Infectious Diseases Society of America</rights><rights>2005 by the Infectious Diseases Society of America 2005</rights><rights>2005 INIST-CNRS</rights><rights>Copyright University of Chicago Press Jul 1, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-91fdce7c566fb810af7427a71a59e7d3424b89a9823e9f3336201c63542c3ab13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30087094$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30087094$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>315,781,785,804,27928,27929,58021,58254</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17000629$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15942901$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Li-Chen</creatorcontrib><creatorcontrib>Fang, Chi-Tai</creatorcontrib><creatorcontrib>Lee, Cha-Ze</creatorcontrib><creatorcontrib>Shun, Chia-Tung</creatorcontrib><creatorcontrib>Wang, Jin-Town</creatorcontrib><title>Genomic Heterogeneity in Klebsiella pneumoniae Strains Is Associated with Primary Pyogenic Liver Abscess and Metastatic Infection</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><addtitle>The Journal of Infectious Diseases</addtitle><description>BackgroundPrimary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease Methods and resultsUsing DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake system (kfu) a phosphoenolpyruvate sugar phosphotransferase system (PTS), and 6 unknown ORFs. The region was more prevalent among tissue-invasive strains (35/46) than among noninvasive strains (19/98) (P<.0001, χ2 test). To test the role played by this region in pathogenesis, 3 different deletion mutants (NTUH-K2044 [Δkfu], K2044 [ΔORF7–9], and K2044 [ΔPTS]) were constructed. Only the ΔkfuABC mutants showed decreased virulence in mice, compared with the wild-type strain. An in vitro assay confirmed the involvement of kfu in iron acquisition. There was a high correlation rate (85%) between the kfu/PTS region and 2 tissue invasion–associated chromosomal regions (allS and magA). Moreover, all 3 regions were present in strains that caused PLA plus endophthalmitis or meningitis ConclusionOur results suggest that chromosomal heterogeneity is present in tissue-invasive K. pneumoniae strains. A genotype containing all 3 regions is strongly associated with PLA and metastatic infection. These regions may serve as convenient markers for the rapid diagnosis of emergent tissue-invasive strains</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Endophthalmitis</subject><subject>Endophthalmitis - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Klebsiella Infections - microbiology</subject><subject>Klebsiella pneumoniae</subject><subject>Klebsiella pneumoniae - genetics</subject><subject>Klebsiella pneumoniae - pathogenicity</subject><subject>Liver abscess</subject><subject>Liver Abscess, Pyogenic - microbiology</subject><subject>Medical sciences</subject><subject>Meningitis, Bacterial - microbiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microarray Analysis</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Open reading frames</subject><subject>Operator regions</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Sepsis - microbiology</subject><subject>Tumors</subject><subject>Virulence</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2LEzEUhgdR3O6q_0CJgt6N5muSyWUt67bYxUVXEW-GTHpG050mNcmovfSfmzJlC4J4lYv34Tk55y2KRwS_JLgWrzjDgqg7xYRUTJZCEHa3mGBMaUlqpU6K0xjXGGPOhLxfnJBKcaowmRS_L8D5jTVoDgmC_woObNoh69DbHtpooe812joYNt5ZDehDCtq6iBYRTWP0xuoEK_TTpm_oKtiNDjt0tdtrsnJpf0BA0zYaiBFpt0KXkHRMOuVw4TowyXr3oLjX6T7Cw8N7Vnx8c349m5fLdxeL2XRZGl6RVCrSrQxIUwnRtTXBupOcSi2JrhTIFeOUt7XSqqYMVMcYExQTI1jFqWG6JeyseDF6t8F_HyCmZmPzz_J-DvwQGyEVYUri_4JE1qKueJ3BZ3-Baz8El5doKGUKc4nro80EH2OArtmOd2oIbvbVNWN1GXxysA3tBlZH7NBVBp4fAB2N7rugnbHxyMncr6B70dOR88P238Mej8w6Jh9uKYZxLbHiOS_H3MYEv25zHW7ynZismvnnL8179uly9lrNmmv2B0YiwqY</recordid><startdate>20050701</startdate><enddate>20050701</enddate><creator>Ma, Li-Chen</creator><creator>Fang, Chi-Tai</creator><creator>Lee, Cha-Ze</creator><creator>Shun, Chia-Tung</creator><creator>Wang, Jin-Town</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20050701</creationdate><title>Genomic Heterogeneity in Klebsiella pneumoniae Strains Is Associated with Primary Pyogenic Liver Abscess and Metastatic Infection</title><author>Ma, Li-Chen ; Fang, Chi-Tai ; Lee, Cha-Ze ; Shun, Chia-Tung ; Wang, Jin-Town</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-91fdce7c566fb810af7427a71a59e7d3424b89a9823e9f3336201c63542c3ab13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Endophthalmitis</topic><topic>Endophthalmitis - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Humans</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Klebsiella Infections - microbiology</topic><topic>Klebsiella pneumoniae</topic><topic>Klebsiella pneumoniae - genetics</topic><topic>Klebsiella pneumoniae - pathogenicity</topic><topic>Liver abscess</topic><topic>Liver Abscess, Pyogenic - microbiology</topic><topic>Medical sciences</topic><topic>Meningitis, Bacterial - microbiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microarray Analysis</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Open reading frames</topic><topic>Operator regions</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Sepsis - microbiology</topic><topic>Tumors</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Li-Chen</creatorcontrib><creatorcontrib>Fang, Chi-Tai</creatorcontrib><creatorcontrib>Lee, Cha-Ze</creatorcontrib><creatorcontrib>Shun, Chia-Tung</creatorcontrib><creatorcontrib>Wang, Jin-Town</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Li-Chen</au><au>Fang, Chi-Tai</au><au>Lee, Cha-Ze</au><au>Shun, Chia-Tung</au><au>Wang, Jin-Town</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic Heterogeneity in Klebsiella pneumoniae Strains Is Associated with Primary Pyogenic Liver Abscess and Metastatic Infection</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2005-07-01</date><risdate>2005</risdate><volume>192</volume><issue>1</issue><spage>117</spage><epage>128</epage><pages>117-128</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>BackgroundPrimary pyogenic liver abscess (PLA) with septic complication by Klebsiella pneumoniae is an emerging infectious disease Methods and resultsUsing DNA microarray hybridization, we identified a 20-kb chromosomal region that contained 15 open-reading frames (ORFs), including an iron-uptake system (kfu) a phosphoenolpyruvate sugar phosphotransferase system (PTS), and 6 unknown ORFs. The region was more prevalent among tissue-invasive strains (35/46) than among noninvasive strains (19/98) (P<.0001, χ2 test). To test the role played by this region in pathogenesis, 3 different deletion mutants (NTUH-K2044 [Δkfu], K2044 [ΔORF7–9], and K2044 [ΔPTS]) were constructed. Only the ΔkfuABC mutants showed decreased virulence in mice, compared with the wild-type strain. An in vitro assay confirmed the involvement of kfu in iron acquisition. There was a high correlation rate (85%) between the kfu/PTS region and 2 tissue invasion–associated chromosomal regions (allS and magA). Moreover, all 3 regions were present in strains that caused PLA plus endophthalmitis or meningitis ConclusionOur results suggest that chromosomal heterogeneity is present in tissue-invasive K. pneumoniae strains. A genotype containing all 3 regions is strongly associated with PLA and metastatic infection. These regions may serve as convenient markers for the rapid diagnosis of emergent tissue-invasive strains</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>15942901</pmid><doi>10.1086/430619</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 2005-07, Vol.192 (1), p.117-128 |
| issn | 0022-1899 1537-6613 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67913970 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Chromosome Mapping Endophthalmitis Endophthalmitis - microbiology Fundamental and applied biological sciences. Psychology Genetic Variation Genome, Bacterial Genomes Genomics Humans Infections Infectious diseases Klebsiella Infections - microbiology Klebsiella pneumoniae Klebsiella pneumoniae - genetics Klebsiella pneumoniae - pathogenicity Liver abscess Liver Abscess, Pyogenic - microbiology Medical sciences Meningitis, Bacterial - microbiology Mice Mice, Inbred BALB C Microarray Analysis Microbiology Miscellaneous Open reading frames Operator regions Plasmids Polymerase chain reaction Sepsis - microbiology Tumors Virulence |
| title | Genomic Heterogeneity in Klebsiella pneumoniae Strains Is Associated with Primary Pyogenic Liver Abscess and Metastatic Infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T13%3A40%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genomic%20Heterogeneity%20in%20Klebsiella%20pneumoniae%20Strains%20Is%20Associated%20with%20Primary%20Pyogenic%20Liver%20Abscess%20and%20Metastatic%20Infection&rft.jtitle=The%20Journal%20of%20infectious%20diseases&rft.au=Ma,%20Li-Chen&rft.date=2005-07-01&rft.volume=192&rft.issue=1&rft.spage=117&rft.epage=128&rft.pages=117-128&rft.issn=0022-1899&rft.eissn=1537-6613&rft.coden=JIDIAQ&rft_id=info:doi/10.1086/430619&rft_dat=%3Cjstor_proqu%3E30087094%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=223904708&rft_id=info:pmid/15942901&rft_jstor_id=30087094&rft_oup_id=10.1086/430619&rfr_iscdi=true |