Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system

Two‐dimensional gel electrophoresis (2‐DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate‐polyacrylamide gel electrophoresis ((SDS‐PAGE)/(Bis (2‐hydroxyethyl) imino‐tris (hydroxymethyl) methane‐HCl, or Bis‐Tris)) have greatly...

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Veröffentlicht in:	Proteomics (Weinheim) 2005-06, Vol.5 (9), p.2309-2314
Hauptverfasser:	Graham, David R. M., Garnham, Christopher P., Fu, Qin, Robbins, Jeffrey, Van Eyk, Jennifer E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Bis-Tris Blood Proteins - chemistry Buffers Casdiac Electrophoresis, Gel, Two-Dimensional - economics Electrophoresis, Gel, Two-Dimensional - methods Fundamental and applied biological sciences. Psychology Heart Ventricles - chemistry HIV-1 - chemistry Human immunodeficiency virus 1 Human immunodeficiency virus type 1 Human viral diseases Humans Hydrogen-Ion Concentration Infectious diseases Medical sciences Miscellaneous Myocardium - chemistry Proteins Proteomics Rabbits Serum Sodium dodecyl sulfate-polyacrylamide gel electrophoresis Tissue Extracts - chemistry Tromethamine - analogs & derivatives Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids
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