Improvements in two-dimensional gel electrophoresis by utilizing a low cost "in-house" neutral pH sodium dodecyl sulfate-polyacrylamide gel electrophoresis system

Two‐dimensional gel electrophoresis (2‐DE) is widely used for initial protein separation in proteomics. Commercial products using neutral pH sodium dodecyl sulfate‐polyacrylamide gel electrophoresis ((SDS‐PAGE)/(Bis (2‐hydroxyethyl) imino‐tris (hydroxymethyl) methane‐HCl, or Bis‐Tris)) have greatly improved this technique, but cost and limited sizes restrict their applications. An “in‐house” system is presented, resulting in better resolution, separation, and new spot visualization and improved resolution when compared to Tris‐HCl gels. Their utility is demonstrated using albumin‐depleted serum samples, rabbit heart left ventricle, and human immunodeficiency virus type 1 (HIV‐1).