Photo-Cross-Linked Small-Molecule Affinity Matrix for Facilitating Forward and Reverse Chemical Genetics

Photo-Cross-Linked Small-Molecule Affinity Matrix for Facilitating Forward and Reverse Chemical Genetics

A photoaffinity reaction has been used to photo‐cross‐link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a...

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Veröffentlicht in:Angewandte Chemie International Edition 2005-06, Vol.44 (23), p.3559-3562
Hauptverfasser: Kanoh, Naoki, Honda, Kaori, Simizu, Siro, Muroi, Makoto, Osada, Hiroyuki
Format: Artikel
Sprache:eng
Schlagworte:
affinity purification
Combinatorial Chemistry Techniques
Cross-Linking Reagents - chemistry
immobilization
Microspheres
molecular targeting
peptides
Photochemistry
Proteins - chemistry
Proteins - isolation & purification
Sepharose - chemistry
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Zusammenfassung:A photoaffinity reaction has been used to photo‐cross‐link small molecules on agarose beads to identify and purify the binding proteins (see picture; PMF: peptide mass fingerprinting). This technology is considered potentially useful for the confirmation of specificity between a small molecule and a protein of interest in the field of reverse chemical genetics.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200462370