Hydride Transfer versus Hydrogen Radical Transfer in Thymidylate Synthase

The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a s...

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Veröffentlicht in:	Journal of the American Chemical Society 2006-05, Vol.128 (17), p.5636-5637
Hauptverfasser:	Hong, Baoyu, Haddad, Majd, Maley, Frank, Jensen, Jan H, Kohen, Amnon
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Fundamental and applied biological sciences. Psychology Hydrogen - chemistry Mechanisms. Catalysis. Electron transfer. Models Molecular biophysics Physical chemistry in biology Thymidylate Synthase - chemistry
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Beschreibung
Zusammenfassung:	The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja060196o