The use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to detect putative breast cancer markers in saliva: a feasibility study
Background: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI) to detect putative br...
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| Veröffentlicht in: | Journal of oral pathology & medicine 2006-05, Vol.35 (5), p.292-300 |
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| creator | Streckfus, Charles F. Bigler, Lenora R. Zwick, Michael |
| description | Background: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI) to detect putative breast cancer markers in saliva.
Methods: Salivary specimens were analyzed as either pooled cancer saliva specimens, or individual specimens from healthy women and women diagnosed with carcinoma of the breast. The specimens were applied to a variety of protein chip arrays, washed extensively to remove unbound analytes and analyzed on a SELDI mass spectrometer.
Results: The results of this initial study suggest that the WCX protein chip array prepared and washed at pH 3.5 yielded the most promising results. Additionally, the analyses revealed a number of proteins that were higher in intensity among the cancer subjects when compared with controls. These salivary proteins were present at the 18, 113, 170, 228 and 287 km/z ranges using SELDI analyses.
Conclusions: The study suggests that saliva may be useful for high‐throughput biomarker discovery. |
| doi_str_mv | 10.1111/j.1600-0714.2006.00427.x |
| format | Article |
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Methods: Salivary specimens were analyzed as either pooled cancer saliva specimens, or individual specimens from healthy women and women diagnosed with carcinoma of the breast. The specimens were applied to a variety of protein chip arrays, washed extensively to remove unbound analytes and analyzed on a SELDI mass spectrometer.
Results: The results of this initial study suggest that the WCX protein chip array prepared and washed at pH 3.5 yielded the most promising results. Additionally, the analyses revealed a number of proteins that were higher in intensity among the cancer subjects when compared with controls. These salivary proteins were present at the 18, 113, 170, 228 and 287 km/z ranges using SELDI analyses.
Conclusions: The study suggests that saliva may be useful for high‐throughput biomarker discovery.</description><identifier>ISSN: 0904-2512</identifier><identifier>EISSN: 1600-0714</identifier><identifier>DOI: 10.1111/j.1600-0714.2006.00427.x</identifier><identifier>PMID: 16630293</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; biomarkers ; breast cancer ; Breast Neoplasms - chemistry ; Breast Neoplasms - diagnosis ; Carcinoma in Situ - chemistry ; Carcinoma in Situ - diagnosis ; Carcinoma, Ductal, Breast - chemistry ; Carcinoma, Ductal, Breast - diagnosis ; Case-Control Studies ; Cell Line, Tumor ; Dentistry ; Feasibility Studies ; Female ; Gynecology. Andrology. Obstetrics ; Humans ; Mammary gland diseases ; Medical sciences ; Neoplasm Proteins - analysis ; Otorhinolaryngology. Stomatology ; Pilot Projects ; Protein Array Analysis ; Saliva - chemistry ; saliva proteome ; Salivary Proteins and Peptides - analysis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; surface-enhanced laser desorption/ionization time-of-flight mass spectrometry ; Tumors</subject><ispartof>Journal of oral pathology & medicine, 2006-05, Vol.35 (5), p.292-300</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4347-5b32efe9ff661c31db45b560386e6b7be9d314ce158d728e67c9c9ff62ac97bb3</citedby><cites>FETCH-LOGICAL-c4347-5b32efe9ff661c31db45b560386e6b7be9d314ce158d728e67c9c9ff62ac97bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0714.2006.00427.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0714.2006.00427.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17677169$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16630293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Streckfus, Charles F.</creatorcontrib><creatorcontrib>Bigler, Lenora R.</creatorcontrib><creatorcontrib>Zwick, Michael</creatorcontrib><title>The use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to detect putative breast cancer markers in saliva: a feasibility study</title><title>Journal of oral pathology & medicine</title><addtitle>J Oral Pathol Med</addtitle><description>Background: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI) to detect putative breast cancer markers in saliva.
Methods: Salivary specimens were analyzed as either pooled cancer saliva specimens, or individual specimens from healthy women and women diagnosed with carcinoma of the breast. The specimens were applied to a variety of protein chip arrays, washed extensively to remove unbound analytes and analyzed on a SELDI mass spectrometer.
Results: The results of this initial study suggest that the WCX protein chip array prepared and washed at pH 3.5 yielded the most promising results. Additionally, the analyses revealed a number of proteins that were higher in intensity among the cancer subjects when compared with controls. These salivary proteins were present at the 18, 113, 170, 228 and 287 km/z ranges using SELDI analyses.
Conclusions: The study suggests that saliva may be useful for high‐throughput biomarker discovery.</description><subject>Biological and medical sciences</subject><subject>biomarkers</subject><subject>breast cancer</subject><subject>Breast Neoplasms - chemistry</subject><subject>Breast Neoplasms - diagnosis</subject><subject>Carcinoma in Situ - chemistry</subject><subject>Carcinoma in Situ - diagnosis</subject><subject>Carcinoma, Ductal, Breast - chemistry</subject><subject>Carcinoma, Ductal, Breast - diagnosis</subject><subject>Case-Control Studies</subject><subject>Cell Line, Tumor</subject><subject>Dentistry</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Mammary gland diseases</subject><subject>Medical sciences</subject><subject>Neoplasm Proteins - analysis</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Pilot Projects</subject><subject>Protein Array Analysis</subject><subject>Saliva - chemistry</subject><subject>saliva proteome</subject><subject>Salivary Proteins and Peptides - analysis</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>surface-enhanced laser desorption/ionization time-of-flight mass spectrometry</subject><subject>Tumors</subject><issn>0904-2512</issn><issn>1600-0714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhiMEokPhFZA3sEtqx4mdIDaoghY0oizKRWwsxzlmPGSS4OO0E56GR8VhRu0WS5Yt-_uOL3-SEEYzFtvZNmOC0pRKVmQ5pSKjtMhltn-QrO42HiYrWtMizUuWnyRPELeUMskL9jg5YUJwmtd8lfy53gCZEMhgCU7eagMp9BvdG2hJpxE8aQEHPwY39Gexu996mZLgdpAONrWd-7EJZKcRCY5ggh92EPxMwhDNEBfIOIXo3ABpPGgMxCzVfVT8T_BIXE9Qd-5GvyKa2Ei4xnUuzATD1M5Pk0dWdwjPjuNp8vnd2-vzy3R9dfH-_M06NQUvZFo2PAcLtbVCMMNZ2xRlUwrKKwGikQ3ULWeFAVZWrcwrENLUZqFzbWrZNPw0eXmoO_rh1wQY1M6hga7TPQwTKiGrOv6ejGB1AI0fED1YNXoX3zIrRtWSjtqqJQS1hKCWdNS_dNQ-qs-PZ0zNDtp78RhHBF4cAY1Gd9bHn3J4z0khJRN15F4fuFvXwfzfF1Afrj7FSdTTg-4wwP5Oj3nEZ3JZqq8fL9S39Rf6vSgqVfK_acK-AA</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Streckfus, Charles F.</creator><creator>Bigler, Lenora R.</creator><creator>Zwick, Michael</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>The use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to detect putative breast cancer markers in saliva: a feasibility study</title><author>Streckfus, Charles F. ; Bigler, Lenora R. ; Zwick, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4347-5b32efe9ff661c31db45b560386e6b7be9d314ce158d728e67c9c9ff62ac97bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biological and medical sciences</topic><topic>biomarkers</topic><topic>breast cancer</topic><topic>Breast Neoplasms - chemistry</topic><topic>Breast Neoplasms - diagnosis</topic><topic>Carcinoma in Situ - chemistry</topic><topic>Carcinoma in Situ - diagnosis</topic><topic>Carcinoma, Ductal, Breast - chemistry</topic><topic>Carcinoma, Ductal, Breast - diagnosis</topic><topic>Case-Control Studies</topic><topic>Cell Line, Tumor</topic><topic>Dentistry</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Mammary gland diseases</topic><topic>Medical sciences</topic><topic>Neoplasm Proteins - analysis</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Pilot Projects</topic><topic>Protein Array Analysis</topic><topic>Saliva - chemistry</topic><topic>saliva proteome</topic><topic>Salivary Proteins and Peptides - analysis</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>surface-enhanced laser desorption/ionization time-of-flight mass spectrometry</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Streckfus, Charles F.</creatorcontrib><creatorcontrib>Bigler, Lenora R.</creatorcontrib><creatorcontrib>Zwick, Michael</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of oral pathology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Streckfus, Charles F.</au><au>Bigler, Lenora R.</au><au>Zwick, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to detect putative breast cancer markers in saliva: a feasibility study</atitle><jtitle>Journal of oral pathology & medicine</jtitle><addtitle>J Oral Pathol Med</addtitle><date>2006-05</date><risdate>2006</risdate><volume>35</volume><issue>5</issue><spage>292</spage><epage>300</epage><pages>292-300</pages><issn>0904-2512</issn><eissn>1600-0714</eissn><abstract>Background: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI) to detect putative breast cancer markers in saliva.
Methods: Salivary specimens were analyzed as either pooled cancer saliva specimens, or individual specimens from healthy women and women diagnosed with carcinoma of the breast. The specimens were applied to a variety of protein chip arrays, washed extensively to remove unbound analytes and analyzed on a SELDI mass spectrometer.
Results: The results of this initial study suggest that the WCX protein chip array prepared and washed at pH 3.5 yielded the most promising results. Additionally, the analyses revealed a number of proteins that were higher in intensity among the cancer subjects when compared with controls. These salivary proteins were present at the 18, 113, 170, 228 and 287 km/z ranges using SELDI analyses.
Conclusions: The study suggests that saliva may be useful for high‐throughput biomarker discovery.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16630293</pmid><doi>10.1111/j.1600-0714.2006.00427.x</doi><tpages>9</tpages></addata></record> |
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| subjects | Biological and medical sciences biomarkers breast cancer Breast Neoplasms - chemistry Breast Neoplasms - diagnosis Carcinoma in Situ - chemistry Carcinoma in Situ - diagnosis Carcinoma, Ductal, Breast - chemistry Carcinoma, Ductal, Breast - diagnosis Case-Control Studies Cell Line, Tumor Dentistry Feasibility Studies Female Gynecology. Andrology. Obstetrics Humans Mammary gland diseases Medical sciences Neoplasm Proteins - analysis Otorhinolaryngology. Stomatology Pilot Projects Protein Array Analysis Saliva - chemistry saliva proteome Salivary Proteins and Peptides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization surface-enhanced laser desorption/ionization time-of-flight mass spectrometry Tumors |
| title | The use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to detect putative breast cancer markers in saliva: a feasibility study |
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