The use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to detect putative breast cancer markers in saliva: a feasibility study
Background: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI) to detect putative br...
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Veröffentlicht in: | Journal of oral pathology & medicine 2006-05, Vol.35 (5), p.292-300 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Background: Technologies are now available enabling saliva to be used to diagnose disease, predict disease progression, and monitor therapeutic efficacy. This pilot study describes the use of surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry (SELDI) to detect putative breast cancer markers in saliva.
Methods: Salivary specimens were analyzed as either pooled cancer saliva specimens, or individual specimens from healthy women and women diagnosed with carcinoma of the breast. The specimens were applied to a variety of protein chip arrays, washed extensively to remove unbound analytes and analyzed on a SELDI mass spectrometer.
Results: The results of this initial study suggest that the WCX protein chip array prepared and washed at pH 3.5 yielded the most promising results. Additionally, the analyses revealed a number of proteins that were higher in intensity among the cancer subjects when compared with controls. These salivary proteins were present at the 18, 113, 170, 228 and 287 km/z ranges using SELDI analyses.
Conclusions: The study suggests that saliva may be useful for high‐throughput biomarker discovery. |
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ISSN: | 0904-2512 1600-0714 |
DOI: | 10.1111/j.1600-0714.2006.00427.x |