Structural Basis for RNA Unwinding by the DEAD-Box Protein Drosophila Vasa

DEAD-box RNA helicases, which regulate various processes involving RNA, have two RecA-like domains as a catalytic core to alter higher-order RNA structures. We determined the 2.2 Å resolution structure of the core of the Drosophila DEAD-box protein Vasa in complex with a single-stranded RNA and an A...

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Veröffentlicht in:Cell 2006-04, Vol.125 (2), p.287-300
Hauptverfasser: Sengoku, Toru, Nureki, Osamu, Nakamura, Akira, Kobayashi, Satoru, Yokoyama, Shigeyuki
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container_end_page 300
container_issue 2
container_start_page 287
container_title Cell
container_volume 125
creator Sengoku, Toru
Nureki, Osamu
Nakamura, Akira
Kobayashi, Satoru
Yokoyama, Shigeyuki
description DEAD-box RNA helicases, which regulate various processes involving RNA, have two RecA-like domains as a catalytic core to alter higher-order RNA structures. We determined the 2.2 Å resolution structure of the core of the Drosophila DEAD-box protein Vasa in complex with a single-stranded RNA and an ATP analog. The ATP analog intensively interacts with both of the domains, thereby bringing them into the closed form, with many interdomain interactions of conserved residues. The bound RNA is sharply bent, avoiding a clash with a conserved α helix in the N-terminal domain. This “wedge” helix should disrupt base pairs by bending one of the strands when a duplex is bound. Mutational analyses indicated that the interdomain interactions couple ATP hydrolysis to RNA unwinding, probably through fine positioning of the duplex relative to the wedge helix. This mechanism, which differs from those for canonical translocating helicases, may enable the targeted modulation of intricate RNA structures.
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subjects Adenosine Triphosphate - analogs & derivatives
Adenosine Triphosphate - metabolism
Amino Acid Sequence
Animals
Crystallography, X-Ray
DEAD-box RNA Helicases
DNA Mutational Analysis
Drosophila
Drosophila melanogaster - enzymology
Drosophila Proteins - chemistry
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Models, Molecular
Molecular Sequence Data
Nucleic Acid Denaturation
Oocytes - cytology
Oocytes - metabolism
Protein Binding
Protein Structure, Tertiary
RNA - chemistry
RNA - metabolism
RNA Helicases - chemistry
RNA Helicases - genetics
RNA Helicases - metabolism
Sequence Alignment
title Structural Basis for RNA Unwinding by the DEAD-Box Protein Drosophila Vasa
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