IMAGING BETA CELL DEVELOPMENT IN REAL-TIME USING PANCREATIC EXPLANTS FROM MICE WITH GREEN FLUORESCENT PROTEIN–LABELED PANCREATIC BETA CELLS

We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.

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Bibliographische Detailangaben
Veröffentlicht in:	In vitro cellular & developmental biology. Animal 2005-01, Vol.41 (1), p.7-11
Hauptverfasser:	GUNAWARDANA, SUBHADRA C, HARA, MANAMI, BELL, GRAEME I, STEVEN HEAD, W, MAGNUSON, MARK A, PISTON, DAVID W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Beta cells Cell Culture Techniques - methods Collagens Cultured cells Developmental biology Fluorescence Gels GFP-labeled Green Fluorescent Proteins - metabolism in vitro culture Islets of Langerhans - cytology Islets of Langerhans - embryology Mice Mice, Transgenic Microscopy, Fluorescence mouse Pancreas Pancreatic cells pancreatic explants real-time Transgenes - genetics Transgenic animals
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Beschreibung
Zusammenfassung:	We present a convenient method for monitoring pancreatic beta cell development in real-time, through in vitro culture of embryonic pancreatic explants from transgenic mice with a genetic tag for insulin-producing beta cells.
ISSN:	1071-2690 1543-706X 1543-706X
DOI:	10.1290/0412080.1