Detection of volatile metabolites produced by bacterial growth in blood culture media by selected ion flow tube mass spectrometry (SIFT-MS)

To achieve faster bacteremia diagnosis, selected ion flow tube mass spectrometry (SIFT-MS) measured metabolic gases in the headspaces of BacT/ALERT® blood culture bottles. Pseudomonas aeruginosa, Streptococcus pneumoniae, Escherichia coli, Staphylococcus aureus and Neisseria meningitidis growth and...

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Veröffentlicht in:	Journal of microbiological methods 2006-05, Vol.65 (2), p.361-365
Hauptverfasser:	Allardyce, Randall A., Langford, Vaughan S., Hill, Alex L., Murdoch, David R.
Format:	Artikel
Sprache:	eng
Schlagworte:	BacT/ALERT Bacteremia - diagnosis Bacteremia - microbiology Bacteria - growth & development Bacteria - metabolism Bacteriological methods and techniques used in bacteriology Bacteriological Techniques - instrumentation Bacteriological Techniques - methods Bacteriology Biological and medical sciences Blood - microbiology Blood culture Culture Media Escherichia coli Fundamental and applied biological sciences. Psychology Humans Mass spectrometry Mass Spectrometry - methods Microbiology Neisseria meningitidis Organic Chemicals - analysis Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Pseudomonas aeruginosa SIFT-MS Staphylococcus aureus Streptococcus pneumoniae Volatilization
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Beschreibung
Zusammenfassung:	To achieve faster bacteremia diagnosis, selected ion flow tube mass spectrometry (SIFT-MS) measured metabolic gases in the headspaces of BacT/ALERT® blood culture bottles. Pseudomonas aeruginosa, Streptococcus pneumoniae, Escherichia coli, Staphylococcus aureus and Neisseria meningitidis growth and trace gas patterns were detected from 10 colony forming units after 6 h.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2005.09.003