Measurement of lactose crystallinity using Raman spectroscopy
Raman spectroscopy (RS) was used to determine the crystallinity of lactose (a commonly used carrier in dry powder inhaler (DPI) formulations). Samples of α-lactose monohydrate and amorphous lactose were prepared using ethanol precipitation and lyophilisation respectively. The anomeric forms were con...
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Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2005-06, Vol.38 (1), p.186-190 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Raman spectroscopy (RS) was used to determine the crystallinity of lactose (a commonly used carrier in dry powder inhaler (DPI) formulations). Samples of α-lactose monohydrate and amorphous lactose were prepared using ethanol precipitation and lyophilisation respectively. The anomeric forms were confirmed using DSC at a rate of 10
°C/min and heated to 250
°C. The Raman spectra of both α-lactose monohydrate and amorphous lactose were obtained. Distinguishable differences were seen between the two spectra including peak areas and intensities. Depolarisation ratios (
ρ) of each form were then determined to identify the crystallinity of the lactose carrier samples. At the prominent Raman bands 865 and 1082
cm
−1, significant differences in
ρ values were observed for crystalline (0.80
±
0.07, 0.89
±
0.06 respectively) and amorphous samples (0.44
±
0.07, 0.51
±
0.10). |
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ISSN: | 0731-7085 1873-264X |
DOI: | 10.1016/j.jpba.2004.12.013 |