Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics

We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter...

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Veröffentlicht in:	Plant and cell physiology 2006-03, Vol.47 (3), p.426-431
Hauptverfasser:	Sun, Hyeon-Jin, Uchii, Sayaka, Watanabe, Shin, Ezura, Hiroshi
Format:	Artikel
Sprache:	eng
Schlagworte:	acetosyringone Agrobacterium tumefaciens Agrobacterium tumefaciens (Rhizobium radiobacter) DNA, Bacterial - genetics EMS EST ethyl methanesulfonate expressed sequence tag Functional genomics Genetic Vectors Genomics - methods Glucuronidase - metabolism GUS Lycopersicon esculentum Lycopersicon esculentum - classification Lycopersicon esculentum - genetics Lycopersicon esculentum L Micro-Tom Murashige and Skoog Plant Roots - genetics Plant Shoots - genetics Plants, Genetically Modified Research Design Rhizobium Rhizobium - genetics Transformation Transformation, Genetic β-glucuronidase
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Zusammenfassung:	We report a highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics. Cotyledon explants of tomato inoculated with Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif[superscript R] harboring the binary vector pIG121Hm generated a mass of chimeric non-transgenic and transgenic adventitious buds. Repeated shoot elongation from the mass of adventitious buds on selection media resulted in the production of multiple transgenic plants that originated from independent transformation events. The transformation efficiency exceeded 40% of the explants. This protocol could become a powerful tool for functional genomics in tomato.
ISSN:	0032-0781 1471-9053
DOI:	10.1093/pcp/pci251