Identification and localization of adrenomedullin-storing cardiac mast cells
Adrenomedullin (AM), a potent vasodilatory hypotensive peptide, is expressed in the heart, where it is known to play a protective action. Light-microscopy immunocytochemistry (ICC) demonstrated the presence of AM immunoreactivity not only in the coronary-vessel wall and ventricular myocytes of the h...
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| Veröffentlicht in: | International journal of molecular medicine 2006-05, Vol.17 (5), p.709-713 |
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| container_title | International journal of molecular medicine |
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| creator | Belloni, Anna Petrelli, Lucia Guidolin, Diego De Toni, Renzo Bova, Sergio Spinazzi, Raffaella Gerosa, Gino Rossi, Gian Nussdorfer, Gastone |
| description | Adrenomedullin (AM), a potent vasodilatory hypotensive peptide, is expressed
in the heart, where it is known to play a protective action. Light-microscopy
immunocytochemistry (ICC) demonstrated the presence of AM immunoreactivity not
only in the coronary-vessel wall and ventricular myocytes of the human and rat
heart, but also in sparse voluminous cells located in the perivascular space.
These cells displayed the same location of toluidine blue-positive mast cells,
and electron microscopy ICC showed AM-immunogold staining over the granules of
rat cardiac mast cells. The incubation of rat left ventricle fragments with the
mast-cell histamine releaser compound 48/80 evidenced groups of AM-positive cells
undergoing degranulation and caused an increase of approximately 50% in the AM
concentration in the incubation medium. Collectively, our findings provide evidence
that at least a subset of cardiac mast cells are able to synthesize and store
AM, and upon stimulation to release it near coronary arterioles and venules. |
| doi_str_mv | 10.3892/ijmm.17.5.709 |
| format | Article |
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in the heart, where it is known to play a protective action. Light-microscopy
immunocytochemistry (ICC) demonstrated the presence of AM immunoreactivity not
only in the coronary-vessel wall and ventricular myocytes of the human and rat
heart, but also in sparse voluminous cells located in the perivascular space.
These cells displayed the same location of toluidine blue-positive mast cells,
and electron microscopy ICC showed AM-immunogold staining over the granules of
rat cardiac mast cells. The incubation of rat left ventricle fragments with the
mast-cell histamine releaser compound 48/80 evidenced groups of AM-positive cells
undergoing degranulation and caused an increase of approximately 50% in the AM
concentration in the incubation medium. Collectively, our findings provide evidence
that at least a subset of cardiac mast cells are able to synthesize and store
AM, and upon stimulation to release it near coronary arterioles and venules.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.17.5.709</identifier><identifier>PMID: 16596251</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Adrenomedullin ; Animals ; Humans ; Immunohistochemistry ; Male ; Mast Cells - chemistry ; Mast Cells - drug effects ; Mast Cells - ultrastructure ; Microscopy, Electron ; Myocardium - chemistry ; Myocardium - cytology ; p-Methoxy-N-methylphenethylamine - pharmacology ; Peptides - analysis ; Rats ; Rats, Sprague-Dawley</subject><ispartof>International journal of molecular medicine, 2006-05, Vol.17 (5), p.709-713</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,5570,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16596251$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Belloni, Anna</creatorcontrib><creatorcontrib>Petrelli, Lucia</creatorcontrib><creatorcontrib>Guidolin, Diego</creatorcontrib><creatorcontrib>De Toni, Renzo</creatorcontrib><creatorcontrib>Bova, Sergio</creatorcontrib><creatorcontrib>Spinazzi, Raffaella</creatorcontrib><creatorcontrib>Gerosa, Gino</creatorcontrib><creatorcontrib>Rossi, Gian</creatorcontrib><creatorcontrib>Nussdorfer, Gastone</creatorcontrib><title>Identification and localization of adrenomedullin-storing cardiac mast cells</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Adrenomedullin (AM), a potent vasodilatory hypotensive peptide, is expressed
in the heart, where it is known to play a protective action. Light-microscopy
immunocytochemistry (ICC) demonstrated the presence of AM immunoreactivity not
only in the coronary-vessel wall and ventricular myocytes of the human and rat
heart, but also in sparse voluminous cells located in the perivascular space.
These cells displayed the same location of toluidine blue-positive mast cells,
and electron microscopy ICC showed AM-immunogold staining over the granules of
rat cardiac mast cells. The incubation of rat left ventricle fragments with the
mast-cell histamine releaser compound 48/80 evidenced groups of AM-positive cells
undergoing degranulation and caused an increase of approximately 50% in the AM
concentration in the incubation medium. Collectively, our findings provide evidence
that at least a subset of cardiac mast cells are able to synthesize and store
AM, and upon stimulation to release it near coronary arterioles and venules.</description><subject>Adrenomedullin</subject><subject>Animals</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mast Cells - chemistry</subject><subject>Mast Cells - drug effects</subject><subject>Mast Cells - ultrastructure</subject><subject>Microscopy, Electron</subject><subject>Myocardium - chemistry</subject><subject>Myocardium - cytology</subject><subject>p-Methoxy-N-methylphenethylamine - pharmacology</subject><subject>Peptides - analysis</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAQhi0EoqUwsqJMLCjBZ8dxPCLER6VKLB3YLMexkaskLnYywK_HVYo63avTo1d3D0K3gAtaC_Lodn1fAC9YwbE4Q0vgAnJSlp_nKQPmOeWsWqCrGHcYE1aK-hItoGKiIgyWaLNuzTA667QanR8yNbRZ57Xq3O-88DZTbTCD7007dZ0b8jj64IavTKvQOqWzXsUx06br4jW6sKqL5uY4V2j7-rJ9fs83H2_r56dNrikhY66wUZq3jdE109gqsBpS1mVDBRfMpL-sFlaYxgJwYlVJQZeEV5iUhjG6Qvdz7T7478nEUfYuHg5Qg_FTlBWvKacCEpjPoA4-xmCs3AfXq_AjAcuDPXmwJ4FLJpO9xN8di6cmvXuij7oS8DADcZ9EudbHE_PvGjhLXRwo_QPbFXq1</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Belloni, Anna</creator><creator>Petrelli, Lucia</creator><creator>Guidolin, Diego</creator><creator>De Toni, Renzo</creator><creator>Bova, Sergio</creator><creator>Spinazzi, Raffaella</creator><creator>Gerosa, Gino</creator><creator>Rossi, Gian</creator><creator>Nussdorfer, Gastone</creator><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Identification and localization of adrenomedullin-storing cardiac mast cells</title><author>Belloni, Anna ; Petrelli, Lucia ; Guidolin, Diego ; De Toni, Renzo ; Bova, Sergio ; Spinazzi, Raffaella ; Gerosa, Gino ; Rossi, Gian ; Nussdorfer, Gastone</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c322t-a0eac7dbec85c0fa1fc1ec8c4b39795e389fc9f9ebf1172fa431c4276024e553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adrenomedullin</topic><topic>Animals</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Mast Cells - chemistry</topic><topic>Mast Cells - drug effects</topic><topic>Mast Cells - ultrastructure</topic><topic>Microscopy, Electron</topic><topic>Myocardium - chemistry</topic><topic>Myocardium - cytology</topic><topic>p-Methoxy-N-methylphenethylamine - pharmacology</topic><topic>Peptides - analysis</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belloni, Anna</creatorcontrib><creatorcontrib>Petrelli, Lucia</creatorcontrib><creatorcontrib>Guidolin, Diego</creatorcontrib><creatorcontrib>De Toni, Renzo</creatorcontrib><creatorcontrib>Bova, Sergio</creatorcontrib><creatorcontrib>Spinazzi, Raffaella</creatorcontrib><creatorcontrib>Gerosa, Gino</creatorcontrib><creatorcontrib>Rossi, Gian</creatorcontrib><creatorcontrib>Nussdorfer, Gastone</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belloni, Anna</au><au>Petrelli, Lucia</au><au>Guidolin, Diego</au><au>De Toni, Renzo</au><au>Bova, Sergio</au><au>Spinazzi, Raffaella</au><au>Gerosa, Gino</au><au>Rossi, Gian</au><au>Nussdorfer, Gastone</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and localization of adrenomedullin-storing cardiac mast cells</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>17</volume><issue>5</issue><spage>709</spage><epage>713</epage><pages>709-713</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>Adrenomedullin (AM), a potent vasodilatory hypotensive peptide, is expressed
in the heart, where it is known to play a protective action. Light-microscopy
immunocytochemistry (ICC) demonstrated the presence of AM immunoreactivity not
only in the coronary-vessel wall and ventricular myocytes of the human and rat
heart, but also in sparse voluminous cells located in the perivascular space.
These cells displayed the same location of toluidine blue-positive mast cells,
and electron microscopy ICC showed AM-immunogold staining over the granules of
rat cardiac mast cells. The incubation of rat left ventricle fragments with the
mast-cell histamine releaser compound 48/80 evidenced groups of AM-positive cells
undergoing degranulation and caused an increase of approximately 50% in the AM
concentration in the incubation medium. Collectively, our findings provide evidence
that at least a subset of cardiac mast cells are able to synthesize and store
AM, and upon stimulation to release it near coronary arterioles and venules.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>16596251</pmid><doi>10.3892/ijmm.17.5.709</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | Spandidos Publications Journals; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adrenomedullin Animals Humans Immunohistochemistry Male Mast Cells - chemistry Mast Cells - drug effects Mast Cells - ultrastructure Microscopy, Electron Myocardium - chemistry Myocardium - cytology p-Methoxy-N-methylphenethylamine - pharmacology Peptides - analysis Rats Rats, Sprague-Dawley |
| title | Identification and localization of adrenomedullin-storing cardiac mast cells |
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