Identification and localization of adrenomedullin-storing cardiac mast cells
Adrenomedullin (AM), a potent vasodilatory hypotensive peptide, is expressed in the heart, where it is known to play a protective action. Light-microscopy immunocytochemistry (ICC) demonstrated the presence of AM immunoreactivity not only in the coronary-vessel wall and ventricular myocytes of the h...
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Veröffentlicht in: | International journal of molecular medicine 2006-05, Vol.17 (5), p.709-713 |
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Zusammenfassung: | Adrenomedullin (AM), a potent vasodilatory hypotensive peptide, is expressed
in the heart, where it is known to play a protective action. Light-microscopy
immunocytochemistry (ICC) demonstrated the presence of AM immunoreactivity not
only in the coronary-vessel wall and ventricular myocytes of the human and rat
heart, but also in sparse voluminous cells located in the perivascular space.
These cells displayed the same location of toluidine blue-positive mast cells,
and electron microscopy ICC showed AM-immunogold staining over the granules of
rat cardiac mast cells. The incubation of rat left ventricle fragments with the
mast-cell histamine releaser compound 48/80 evidenced groups of AM-positive cells
undergoing degranulation and caused an increase of approximately 50% in the AM
concentration in the incubation medium. Collectively, our findings provide evidence
that at least a subset of cardiac mast cells are able to synthesize and store
AM, and upon stimulation to release it near coronary arterioles and venules. |
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ISSN: | 1107-3756 1791-244X |
DOI: | 10.3892/ijmm.17.5.709 |