Ultra-performance liquid chromatography coupled to linear ion trap mass spectrometry for the identification of drug metabolites in biological samples

The coupling of ultra‐performance liquid chromatography, operating at elevated pressures, to a linear ion trap mass spectrometer provides a high‐performance system suitable for drug metabolite characterisation. This system demonstrates improved chromatographic efficiency and sensitivity and at the s...

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Veröffentlicht in:Rapid communications in mass spectrometry 2006-01, Vol.20 (8), p.1351-1360
Hauptverfasser: Dear, G. J., James, A. D., Sarda, S.
Format: Artikel
Sprache:eng
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Zusammenfassung:The coupling of ultra‐performance liquid chromatography, operating at elevated pressures, to a linear ion trap mass spectrometer provides a high‐performance system suitable for drug metabolite characterisation. This system demonstrates improved chromatographic efficiency and sensitivity and at the same time provides diagnostic MSn data often critical for metabolite structural assignment. The linear ion trap was capable of dealing with the high chromatographic efficiencies and hence narrow peak widths associated with 1.7 µm particle‐packed column separations. Polarity switching and data‐dependent MSn data were generated with ease, and applied to the identification of metabolites found in human plasma. Copyright © 2006 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.2462