Amelioration of the Effects of Citrobacter rodentium Infection in Mice by Pretreatment with Probiotics

Background. Citrobacter rodentium is a naturally occurring murine pathogen that causes colonic epithelialcell hyperplasia, disrupts the colonic mucosa, and elicits a predominantly T helper 1 cellular immune response; it thereby serves as a model for the study of mechanisms of disease induced by huma...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The Journal of infectious diseases 2005-06, Vol.191 (12), p.2106-2117
Hauptverfasser: Johnson-Henry, Kathene C., Nadjafi, Maral, Avitzur, Yaron, Mitchell, David J., Ngan, Bo-Yee, Galindo-Mata, Esther, Jones, Nicola L., Sherman, Philip M.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Background. Citrobacter rodentium is a naturally occurring murine pathogen that causes colonic epithelialcell hyperplasia, disrupts the colonic mucosa, and elicits a predominantly T helper 1 cellular immune response; it thereby serves as a model for the study of mechanisms of disease induced by human attaching-effacing pathogens. We sought to determine whether pretreatment of mice with a mixture of Lactobacillus rhamnosus and L. acidophilus probiotics would attenuate C. rodentium—induced colonic disease in mice. Methods. Mice were administered sterile drinking water, probiotics (109 cfu/mL) in sterile drinking water, maltodextrin in sterile drinking water, orogastric C. rodentium (107 cfu in 0.1 mL), or maltodextrin in sterile drinking water for 1 week before C. rodentium infection, or they were pretreated with probiotics (109 cfu/mL) for 1 week before challenge with C. rodentium. Results. Mice that received viable probiotics remained healthy. C. rodentium infection elicited mucosal inflammation, epithelial-cell hyperplasia, apoptosis in the colon, and interferon (IFN)-γ production by splenocytes. Pretreatment with probiotics decreased levels of all but IFN-γ production. Conclusions. Pretreatment with probiotics attenuates the effects of C. rodentium infection in mice. Understanding the mechanism of these beneficial effects will aid in determining the efficacy of probiotics in preventing infection with related attaching-effacing enteric pathogens in humans.
ISSN:0022-1899
1537-6613
DOI:10.1086/430318