Pseudomonas otitidis sp. nov., isolated from patients with otic infections

Alcon Research Ltd, 6201 South Freeway, R0-17, Fort Worth, TX 76134-2099, USA Correspondence David W. Stroman David.Stroman{at}alconlabs.com A novel Pseudomonas species, for which the name Pseudomonas otitidis sp. nov. is proposed, was identified from clinical specimens of infected human ears. Forty-one pseudomonads (34 from patients with acute otitis externa, six from patients with acute otitis media with otorrhoea and one from a patient with chronic suppurative otitis media) were recovered that did not match any known species. On the basis of genetic analyses and biochemical characterization, these isolates were shown to belong to the genus Pseudomonas . 16S rRNA gene sequence analysis and DNA–DNA hybridization studies indicated that this novel bacterium is closely related to, but different from, Pseudomonas aeruginosa . A description of this species is based on polyphasic studies of 11 clinical isolates. The type strain of Pseudomonas otitidis is MCC10330 T (=ATCC BAA-1130 T =DSM 17224 T ). Abbreviations: MIC, minimal inhibitory concentration The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of MCC10330 T is AY953147 . Tables detailing the biochemical and phenotypic characteristics of individual novel strains and the cellular fatty acid composition of the novel strains and related Pseudomonas species are available as supplementary material in IJSEM Online.