Proteomic identification of Ku70/Ku80 autoantigen recognized by monoclonal antibody against hepatocellular carcinoma

A murine monoclonal antibody (mAb), CLD3 (IgG1,κ), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tiss...

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Veröffentlicht in:	Proteomics (Weinheim) 2005-05, Vol.5 (7), p.1980-1986
Hauptverfasser:	Luk, John M., Su, Yvonne C. F., Lam, Stanley C. T., Lee, Carol K., Hu, Mei Yu, He, Qing Yu, Lau, George K., Wong, Fred W. S., Fan, Sheung-Tat
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Antibodies, Monoclonal - metabolism Antibodies, Neoplasm - metabolism Antigens, Nuclear - immunology Antigens, Nuclear - metabolism Applied microbiology Binding Sites, Antibody Biological and medical sciences Carcinoma, Hepatocellular - chemistry Carcinoma, Hepatocellular - immunology DNA-Binding Proteins - immunology DNA-Binding Proteins - metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gastroenterology. Liver. Pancreas. Abdomen Hepatocellular carcinoma Humans Immunohistochemistry Ku Autoantigen Liver Neoplasms - immunology Liver Neoplasms - metabolism Liver. Biliary tract. Portal circulation. Exocrine pancreas Medical sciences Microbiology Miscellaneous Molecular Sequence Data Monoclonal antibody Proteins Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tumor marker Tumors Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Zusammenfassung:	A murine monoclonal antibody (mAb), CLD3 (IgG1,κ), was generated against hepatocellular carcinoma (HCC). Both immunofluorescence and immunohistochemical assays indicated the reactivity of CLD3 mAb localized at the nucleus and/or cytoplasm of tumorigenic HCC cell lines as well as in liver cancer tissues. By immunoprecipitation and using the matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry approach, the antigenic specificity of CLD3 was determined to be heterodimeric Ku70 and Ku80 autoantigen, which was confirmed by Western blotting.
ISSN:	1615-9853 1615-9861
DOI:	10.1002/pmic.200401084