Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates

1 Institut für Mikrobiologie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany 2 Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26–32 (IFZ), D-35392 Giessen, Germany 3 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Veterinärplatz 1, A-1210 Wien, Austria Correspondence Andreas Stolz Andreas.Stolz{at}imb.Uni-Stuttgart.de The taxonomic position of a Pseudomonas -like strain, designated BN9 T , was investigated. This strain had previously been isolated as a 5-aminosalicylate-degrading organism from a 6-aminonaphthalene-2-sulphonate-degrading mixed bacterial culture. Previously, detection of ubiquinone Q-8, a polyamine pattern with putrescine, spermidine and 2-hydroxyputrescine as the major polyamines, and partial 16S rRNA gene sequencing had suggested that strain BN9 T belongs to the ‘ Betaproteobacteria ’. This was supported by sequencing the 16S rRNA gene, which demonstrated 94–96 % sequence similarity to different species of the genera Achromobacter , Alcaligenes and Bordetella , and suggested that strain BN9 T represents a member of the family Alcaligenaceae . Different methods for the construction of phylogenetic dendrograms placed the strain separately from all other genera within the Alcaligenaceae . Fatty acid analysis demonstrated the presence of high concentrations of C 19 : 0 cyclo 8 c . On the basis of low 16S rRNA gene sequence similarity to other members of the Alcaligenaceae , fatty acid and polar lipid profiles, and other unique phenotypic properties of strain BN9 T , the creation of a new genus and species with the name Pusillimonas noertemannii gen. nov., sp. nov. is proposed. The type strain is BN9 T (=DSM 10065 T =NCIMB 14020 T ). Published online ahead of print on 3 December 2004 as DOI 10.1099/ijs.0.63466-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Pusillimonas noertemannii BN9 T is AY695828 . Two-dimensional chromatograph of polar lipids of different members of the Alcaligenaceae is available as supplementary material in IJSEM Online.