New Embedded Process for Compartmentalized Cell-Free Protein Expression and On-line Detection in Microfluidic Devices

Just a drop. In the field of biological research and biotechnology, fast and efficient screening for particular properties of minute quantities down to single‐particle level has become increasingly important. This work describes the implementation of a lab‐on‐chip device to generate small reaction containers, that is, a homodisperse water‐in‐oil emulsion, and cell‐free expression of the green fluorescent protein inside the aqueous droplets. Detection of the autofluorescent protein was performed by confocal fluorescence spectroscopy.