A SPECIFIC PRIMER PAIR FOR THE DIAGNOSIS AND IDENTIFICATION OF ACANTHAMOEBA ASTRONYXIS BY RANDOM AMPLIFIED POLYMORPHIC DNA– POLYMERASE CHAIN REACTION

Random amplified polymorphic DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that is useful for species identification. In this study, a distinctive 485-bp band in Acanthamoeba astronyxis band patterns was found, using th...

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Veröffentlicht in:The Journal of parasitology 2005-02, Vol.91 (1), p.122-126
Hauptverfasser: Ortega-Rivas, A, Lorenzo-Morales, J, Martínez, E, Villa, M, Clavel, A, Valladares, B, del Castillo, A
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Sprache:eng
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Zusammenfassung:Random amplified polymorphic DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that is useful for species identification. In this study, a distinctive 485-bp band in Acanthamoeba astronyxis band patterns was found, using the OPC20 primer (ACTTCGCCAC). The band specificity was confirmed by hybridization, using it as a probe, against all OPC20 amplifications from different Acanthamoeba species. Once the fragment was sequenced, we used it to design a specific primer pair that was useful for the identification of different isolates as A. astronyxis species.
ISSN:0022-3395
1937-2345
DOI:10.1645/GE-3301