A SPECIFIC PRIMER PAIR FOR THE DIAGNOSIS AND IDENTIFICATION OF ACANTHAMOEBA ASTRONYXIS BY RANDOM AMPLIFIED POLYMORPHIC DNA– POLYMERASE CHAIN REACTION
Random amplified polymorphic DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that is useful for species identification. In this study, a distinctive 485-bp band in Acanthamoeba astronyxis band patterns was found, using th...
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Veröffentlicht in: | The Journal of parasitology 2005-02, Vol.91 (1), p.122-126 |
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Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Random amplified polymorphic DNA (RAPD) is a useful tool for species identification. The obtained band patterns can be used for specific primer pair design that is useful for species identification. In this study, a distinctive 485-bp band in Acanthamoeba astronyxis band patterns was found, using the OPC20 primer (ACTTCGCCAC). The band specificity was confirmed by hybridization, using it as a probe, against all OPC20 amplifications from different Acanthamoeba species. Once the fragment was sequenced, we used it to design a specific primer pair that was useful for the identification of different isolates as A. astronyxis species. |
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ISSN: | 0022-3395 1937-2345 |
DOI: | 10.1645/GE-3301 |