Label-Free Affinity Assays by Rapid Detection of Immune Complexes in Submicrometer Pores

Resistance movement: A submicrometer conical pore was used for the single‐aggregate detection and characterization of immune complexes consisting of proteins such as staphylococcal enterotoxin B and antibodies. The developed assay is rapid, label‐free, requires no immobilization or modification of t...

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Veröffentlicht in:	Angewandte Chemie International Edition 2006-03, Vol.45 (14), p.2281-2285
Hauptverfasser:	Uram, Jeffrey D., Ke, Kevin, Hunt, Alan J., Mayer, Michael
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - immunology Antigen-Antibody Complex - metabolism Baculoviridae - immunology Biological Assay Coulter counting Enterotoxins - immunology Enterotoxins - metabolism immunoassays nanomachining nanotechnology Particle Size
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Resistance movement: A submicrometer conical pore was used for the single‐aggregate detection and characterization of immune complexes consisting of proteins such as staphylococcal enterotoxin B and antibodies. The developed assay is rapid, label‐free, requires no immobilization or modification of the antibody or antigen, and can be performed with antibodies or proteins in serum.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200502862