Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins

Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins

Quantitative LC-MS/MS assays were designed for tryptic peptides representing 53 high and medium abundance proteins in human plasma using a multiplexed multiple reaction monitoring (MRM) approach. Of these, 47 produced acceptable quantitative data, demonstrating within-run coefficients of variation (...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Molecular & cellular proteomics 2006-04, Vol.5 (4), p.573-588
Hauptverfasser: Anderson, Leigh, Hunter, Christie L.
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Blood Proteins - analysis
Chromatography, Liquid
Humans
Molecular Sequence Data
Peptide Mapping
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Quantitative LC-MS/MS assays were designed for tryptic peptides representing 53 high and medium abundance proteins in human plasma using a multiplexed multiple reaction monitoring (MRM) approach. Of these, 47 produced acceptable quantitative data, demonstrating within-run coefficients of variation (CVs) (n = 10) of 2–22% (78% of assays had CV
ISSN:1535-9476
1535-9484
DOI:10.1074/mcp.M500331-MCP200