Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline

Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50 μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was perfor...

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Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2006-05, Vol.16 (9), p.2407-2410
Hauptverfasser:	Adamczyk, Maciej, Brashear, R. Jeffrey, Mattingly, Phillip G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridines - chemistry Acridinium-9-carboxamide Analysis Betaine - chemical synthesis Biological and medical sciences Chemiluminescence Choline Choline - blood Choline - chemistry General pharmacology Homogeneous assay Humans Hydrogen Peroxide - analysis Investigative techniques, diagnostic techniques (general aspects) Luminescent Measurements - methods Medical sciences Miscellaneous. Technology Molecular Structure Oxidation-Reduction Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pharmacology. Drug treatments Sensitivity and Specificity Time Factors
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Zusammenfassung:	Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50 μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1 μL) and analysis time (
ISSN:	0960-894X 1464-3405
DOI:	10.1016/j.bmcl.2006.01.114