The Effect of Interleukin-17 on the Proliferation and Invasion of JEG-3 Human Choriocarcinoma Cells
Problem As there has been a study in mice showing the expression of IL‐17 by decidual cells and the status of IL‐17 receptor expression in human pregnancy is not known, we hypothesized that IL‐17 may regulate human trophoblast proliferation and invasion. Method of study JEG‐3 cell line was used as...
Gespeichert in:
| Veröffentlicht in: | American journal of reproductive immunology (1989) 2006-04, Vol.55 (4), p.291-300 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 300 |
|---|---|
| container_issue | 4 |
| container_start_page | 291 |
| container_title | American journal of reproductive immunology (1989) |
| container_volume | 55 |
| creator | Pongcharoen, Sutatip Niumsup, Pannika Sanguansermsri, Donruedee Supalap, Kwansuda Butkhamchot, Puntharee |
| description | Problem As there has been a study in mice showing the expression of IL‐17 by decidual cells and the status of IL‐17 receptor expression in human pregnancy is not known, we hypothesized that IL‐17 may regulate human trophoblast proliferation and invasion.
Method of study JEG‐3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL‐17 receptor protein and mRNA, respectively. The effects of IL‐17 on JEG‐3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively.
Results IL‐17 increased the invasive capacity of JEG‐3 cells but had no effect on the proliferation and multinucleated formation of JEG‐3 cells.
Conclusion In this JEG‐3 cell model of human trophoblast, the IL‐17R and IL‐17 may have a regulatory role in trophoblast invasion. |
| doi_str_mv | 10.1111/j.1600-0897.2006.00366.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67744247</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17181707</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5016-b823f9ca831294824aacf1d7135a13d373b0fd8565c18d62b472ab25cdc361f53</originalsourceid><addsrcrecordid>eNqNkctu3CAUhlHUqLm0r1Cx6s4OmOtI3USjyWRy6UVNlewQxqAwsc0E7Hby9sWdUbps2XA45_8O8B8AIEYlzutsXWKOUIHkTJQVQrxEiHBebg_A8WvhTY4R5YWgSB6Bk5TWCOU8EW_BEeaMEELxMTB3jxYunLNmgMHBVT_Y2NrxyfcFFjD0cMj1rzG03tmoB58zum-y7qdO0yEzV4tlQeDl2Okezh9D9MHoaHwfOg3ntm3TO3DodJvs-_1-Cn5cLO7ml8XNl-Vqfn5TGIYwL2pZETczWhJczaisqNbG4UZgwjQmDRGkRq6RjDODZcOrmopK1xUzjSEcO0ZOwcdd300Mz6NNg-p8MvkFurdhTIoLQWlFxT-FWGCJBZqEcic0MaQUrVOb6DsdXxRGapqEWqvJcDUZrqZJqD-TUNuMftjfMdadbf6Ce-uz4NNO8Mu39uW_G6vzq1UOMl7scJ8Gu33FdXzK_ySCqfvPS3X7_dv98vrhWjHyG-cppGg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17181707</pqid></control><display><type>article</type><title>The Effect of Interleukin-17 on the Proliferation and Invasion of JEG-3 Human Choriocarcinoma Cells</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Pongcharoen, Sutatip ; Niumsup, Pannika ; Sanguansermsri, Donruedee ; Supalap, Kwansuda ; Butkhamchot, Puntharee</creator><creatorcontrib>Pongcharoen, Sutatip ; Niumsup, Pannika ; Sanguansermsri, Donruedee ; Supalap, Kwansuda ; Butkhamchot, Puntharee</creatorcontrib><description>Problem As there has been a study in mice showing the expression of IL‐17 by decidual cells and the status of IL‐17 receptor expression in human pregnancy is not known, we hypothesized that IL‐17 may regulate human trophoblast proliferation and invasion.
Method of study JEG‐3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL‐17 receptor protein and mRNA, respectively. The effects of IL‐17 on JEG‐3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively.
Results IL‐17 increased the invasive capacity of JEG‐3 cells but had no effect on the proliferation and multinucleated formation of JEG‐3 cells.
Conclusion In this JEG‐3 cell model of human trophoblast, the IL‐17R and IL‐17 may have a regulatory role in trophoblast invasion.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.2006.00366.x</identifier><identifier>PMID: 16533341</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Cell Line, Tumor ; Cell Proliferation ; Choriocarcinoma - immunology ; Choriocarcinoma - metabolism ; Choriocarcinoma - pathology ; Humans ; Immunohistochemistry ; Interleukin-17 ; Interleukin-17 - physiology ; interleukin-17 receptor ; JEG-3 choriocarcinoma cells ; Neoplasm Invasiveness ; Receptors, Interleukin - biosynthesis ; Receptors, Interleukin - genetics ; Receptors, Interleukin - physiology ; Receptors, Interleukin-17 ; RNA, Messenger - biosynthesis ; Trophoblasts - immunology ; Trophoblasts - metabolism ; Trophoblasts - pathology</subject><ispartof>American journal of reproductive immunology (1989), 2006-04, Vol.55 (4), p.291-300</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5016-b823f9ca831294824aacf1d7135a13d373b0fd8565c18d62b472ab25cdc361f53</citedby><cites>FETCH-LOGICAL-c5016-b823f9ca831294824aacf1d7135a13d373b0fd8565c18d62b472ab25cdc361f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0897.2006.00366.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0897.2006.00366.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16533341$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pongcharoen, Sutatip</creatorcontrib><creatorcontrib>Niumsup, Pannika</creatorcontrib><creatorcontrib>Sanguansermsri, Donruedee</creatorcontrib><creatorcontrib>Supalap, Kwansuda</creatorcontrib><creatorcontrib>Butkhamchot, Puntharee</creatorcontrib><title>The Effect of Interleukin-17 on the Proliferation and Invasion of JEG-3 Human Choriocarcinoma Cells</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem As there has been a study in mice showing the expression of IL‐17 by decidual cells and the status of IL‐17 receptor expression in human pregnancy is not known, we hypothesized that IL‐17 may regulate human trophoblast proliferation and invasion.
Method of study JEG‐3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL‐17 receptor protein and mRNA, respectively. The effects of IL‐17 on JEG‐3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively.
Results IL‐17 increased the invasive capacity of JEG‐3 cells but had no effect on the proliferation and multinucleated formation of JEG‐3 cells.
Conclusion In this JEG‐3 cell model of human trophoblast, the IL‐17R and IL‐17 may have a regulatory role in trophoblast invasion.</description><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Choriocarcinoma - immunology</subject><subject>Choriocarcinoma - metabolism</subject><subject>Choriocarcinoma - pathology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Interleukin-17</subject><subject>Interleukin-17 - physiology</subject><subject>interleukin-17 receptor</subject><subject>JEG-3 choriocarcinoma cells</subject><subject>Neoplasm Invasiveness</subject><subject>Receptors, Interleukin - biosynthesis</subject><subject>Receptors, Interleukin - genetics</subject><subject>Receptors, Interleukin - physiology</subject><subject>Receptors, Interleukin-17</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Trophoblasts - immunology</subject><subject>Trophoblasts - metabolism</subject><subject>Trophoblasts - pathology</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu3CAUhlHUqLm0r1Cx6s4OmOtI3USjyWRy6UVNlewQxqAwsc0E7Hby9sWdUbps2XA45_8O8B8AIEYlzutsXWKOUIHkTJQVQrxEiHBebg_A8WvhTY4R5YWgSB6Bk5TWCOU8EW_BEeaMEELxMTB3jxYunLNmgMHBVT_Y2NrxyfcFFjD0cMj1rzG03tmoB58zum-y7qdO0yEzV4tlQeDl2Okezh9D9MHoaHwfOg3ntm3TO3DodJvs-_1-Cn5cLO7ml8XNl-Vqfn5TGIYwL2pZETczWhJczaisqNbG4UZgwjQmDRGkRq6RjDODZcOrmopK1xUzjSEcO0ZOwcdd300Mz6NNg-p8MvkFurdhTIoLQWlFxT-FWGCJBZqEcic0MaQUrVOb6DsdXxRGapqEWqvJcDUZrqZJqD-TUNuMftjfMdadbf6Ce-uz4NNO8Mu39uW_G6vzq1UOMl7scJ8Gu33FdXzK_ySCqfvPS3X7_dv98vrhWjHyG-cppGg</recordid><startdate>200604</startdate><enddate>200604</enddate><creator>Pongcharoen, Sutatip</creator><creator>Niumsup, Pannika</creator><creator>Sanguansermsri, Donruedee</creator><creator>Supalap, Kwansuda</creator><creator>Butkhamchot, Puntharee</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200604</creationdate><title>The Effect of Interleukin-17 on the Proliferation and Invasion of JEG-3 Human Choriocarcinoma Cells</title><author>Pongcharoen, Sutatip ; Niumsup, Pannika ; Sanguansermsri, Donruedee ; Supalap, Kwansuda ; Butkhamchot, Puntharee</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5016-b823f9ca831294824aacf1d7135a13d373b0fd8565c18d62b472ab25cdc361f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Choriocarcinoma - immunology</topic><topic>Choriocarcinoma - metabolism</topic><topic>Choriocarcinoma - pathology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Interleukin-17</topic><topic>Interleukin-17 - physiology</topic><topic>interleukin-17 receptor</topic><topic>JEG-3 choriocarcinoma cells</topic><topic>Neoplasm Invasiveness</topic><topic>Receptors, Interleukin - biosynthesis</topic><topic>Receptors, Interleukin - genetics</topic><topic>Receptors, Interleukin - physiology</topic><topic>Receptors, Interleukin-17</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Trophoblasts - immunology</topic><topic>Trophoblasts - metabolism</topic><topic>Trophoblasts - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pongcharoen, Sutatip</creatorcontrib><creatorcontrib>Niumsup, Pannika</creatorcontrib><creatorcontrib>Sanguansermsri, Donruedee</creatorcontrib><creatorcontrib>Supalap, Kwansuda</creatorcontrib><creatorcontrib>Butkhamchot, Puntharee</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pongcharoen, Sutatip</au><au>Niumsup, Pannika</au><au>Sanguansermsri, Donruedee</au><au>Supalap, Kwansuda</au><au>Butkhamchot, Puntharee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Interleukin-17 on the Proliferation and Invasion of JEG-3 Human Choriocarcinoma Cells</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2006-04</date><risdate>2006</risdate><volume>55</volume><issue>4</issue><spage>291</spage><epage>300</epage><pages>291-300</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem As there has been a study in mice showing the expression of IL‐17 by decidual cells and the status of IL‐17 receptor expression in human pregnancy is not known, we hypothesized that IL‐17 may regulate human trophoblast proliferation and invasion.
Method of study JEG‐3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL‐17 receptor protein and mRNA, respectively. The effects of IL‐17 on JEG‐3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively.
Results IL‐17 increased the invasive capacity of JEG‐3 cells but had no effect on the proliferation and multinucleated formation of JEG‐3 cells.
Conclusion In this JEG‐3 cell model of human trophoblast, the IL‐17R and IL‐17 may have a regulatory role in trophoblast invasion.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16533341</pmid><doi>10.1111/j.1600-0897.2006.00366.x</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1046-7408 |
| ispartof | American journal of reproductive immunology (1989), 2006-04, Vol.55 (4), p.291-300 |
| issn | 1046-7408 1600-0897 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67744247 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Cell Line, Tumor Cell Proliferation Choriocarcinoma - immunology Choriocarcinoma - metabolism Choriocarcinoma - pathology Humans Immunohistochemistry Interleukin-17 Interleukin-17 - physiology interleukin-17 receptor JEG-3 choriocarcinoma cells Neoplasm Invasiveness Receptors, Interleukin - biosynthesis Receptors, Interleukin - genetics Receptors, Interleukin - physiology Receptors, Interleukin-17 RNA, Messenger - biosynthesis Trophoblasts - immunology Trophoblasts - metabolism Trophoblasts - pathology |
| title | The Effect of Interleukin-17 on the Proliferation and Invasion of JEG-3 Human Choriocarcinoma Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T15%3A22%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Effect%20of%20Interleukin-17%20on%20the%20Proliferation%20and%20Invasion%20of%20JEG-3%20Human%20Choriocarcinoma%20Cells&rft.jtitle=American%20journal%20of%20reproductive%20immunology%20(1989)&rft.au=Pongcharoen,%20Sutatip&rft.date=2006-04&rft.volume=55&rft.issue=4&rft.spage=291&rft.epage=300&rft.pages=291-300&rft.issn=1046-7408&rft.eissn=1600-0897&rft_id=info:doi/10.1111/j.1600-0897.2006.00366.x&rft_dat=%3Cproquest_cross%3E17181707%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17181707&rft_id=info:pmid/16533341&rfr_iscdi=true |