The Effect of Interleukin-17 on the Proliferation and Invasion of JEG-3 Human Choriocarcinoma Cells

Problem As there has been a study in mice showing the expression of IL‐17 by decidual cells and the status of IL‐17 receptor expression in human pregnancy is not known, we hypothesized that IL‐17 may regulate human trophoblast proliferation and invasion. Method of study JEG‐3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL‐17 receptor protein and mRNA, respectively. The effects of IL‐17 on JEG‐3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively. Results IL‐17 increased the invasive capacity of JEG‐3 cells but had no effect on the proliferation and multinucleated formation of JEG‐3 cells. Conclusion In this JEG‐3 cell model of human trophoblast, the IL‐17R and IL‐17 may have a regulatory role in trophoblast invasion.