Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly

Detection of DNA hybridization by ABEI electrochemiluminescence in DNA-chip compatible assembly

The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been...

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Veröffentlicht in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2005-04, Vol.66 (1), p.139-143
Hauptverfasser: Calvo-Muñoz, M.-L., Dupont-Filliard, A., Billon, M., Guillerez, S., Bidan, G., Marquette, C., Blum, L.
Format: Artikel
Sprache:eng
Schlagworte:
ABEI
Detection
DNA
ECL
Electrochemistry - methods
Electrodes
Luminescent Measurements - methods
Luminol - analogs & derivatives
Luminol - chemistry
Membranes, Artificial
Nucleic Acid Hybridization - methods
Oligonucleotide Array Sequence Analysis - methods
Polymers
Polypyrrole
Pyrroles
Sensor
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Zusammenfassung:The electrochemiluminescence (ECL) of a luminol derivate (ABEI) generated both by a carbon electrode and a polypyrrole-coated carbon electrode was examined. It was found that the polypyrrole film (ppy) did not inhibit the ECL. After that, ABEI anchored on a single stranded DNA target (ODNt) has been used for the ECL detection of the hybridization between a complementary single stranded DNA probe (ODNp) covalently linked to a polypyrrole support and the ODNt. The ECL detection has been performed using a DNA sensor having a low surface concentration of ODNp probes, constituted of a polypyrrole copolymer electrosynthesized from a pyrrole-ODNp/pyrrole monomer ratio of 1/20,000.
ISSN:1567-5394
DOI:10.1016/j.bioelechem.2004.04.009