Putative virulence-related genes in Vibrio anguillarum identified by random genome sequencing

The genome of Vibrio anguillarum strain H775‐3 was partially determined by a random sequencing procedure. A total of 2300 clones, 2100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clone...

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Veröffentlicht in:	Journal of fish diseases 2006-03, Vol.29 (3), p.157-166
Hauptverfasser:	Rodkhum, C, Hirono, I, Stork, M, Di Lorenzo, M, Crosa, J H, Aoki, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Capsules - genetics colonization factors Databases, Nucleic Acid exotoxins Exotoxins - genetics Fish Diseases - microbiology Genome, Bacterial - genetics Genome, Bacterial - physiology Genomic Library Genomics - methods lipopolysaccharide Molecular Sequence Data random genome sequencing Sequence Homology, Amino Acid Vibrio - genetics Vibrio - pathogenicity Vibrio anguillarum virulence Virulence Factors - classification Virulence Factors - genetics
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Zusammenfassung:	The genome of Vibrio anguillarum strain H775‐3 was partially determined by a random sequencing procedure. A total of 2300 clones, 2100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence‐related genes were identified, 36 of which are novel in V. anguillarum, including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes.
ISSN:	0140-7775 1365-2761
DOI:	10.1111/j.1365-2761.2006.00692.x