Interfacial energetics of blood plasma and serum adsorption to a hydrophobic self-assembled monolayer surface

Interfacial energetics of blood plasma and serum adsorption to a hydrophobic, methyl-terminated self-assembled monolayer (SAM) surface (solid–liquid SL interface) are shown to be essentially the same as to the buffer–air interface (liquid–vapor LV interface). Specifically, spreading pressure ( Π a) isotherms scaled on a w/v concentration basis constructed from advancing contact angles ( θ a) of serially diluted plasma/serum derived from four different mammalian species (bovine, equine, human, and ovine) on the SAM surface are not resolvable at the 99% confidence level and furthermore are found to be strikingly similar to isotherms of purified human-blood proteins. Maximum advancing spreading pressures Π a max for protein mixtures fall within a relatively narrow 17< Π a max