Rapid protocol for electroporation of Clostridium perfringens

A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transform...

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Veröffentlicht in:	Journal of microbiological methods 2005-07, Vol.62 (1), p.125-127
Hauptverfasser:	Jirásková, Alena, Vítek, Libor, Fevery, Johan, Ruml, Tomáš, Branny, Pavel
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Clostridium perfringens Clostridium perfringens - genetics Electroporation Electroporation - methods Fundamental and applied biological sciences. Psychology Genetics Microbiology Plasmids - administration & dosage Plasmids - genetics Transformation efficiency Transformation, Bacterial - genetics
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Beschreibung
Zusammenfassung:	A rapid and simple method has been developed for the electroporation of Clostridium perfringens with plasmid DNA. The new improvements, harvesting cells early in the logarithmic stage of growth, keeping the cells at room temperature and the absence of post-shock incubation on ice increased transformation efficiency by one order of magnitude.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2005.01.012