Faster axial band dispersion in a monolithic silica column than in a particle-packed column

The contribution of molecular diffusion to peak broadening was studied in a reversed-phase HPLC system, consisting of a monolithic silica C 18 column and methanol–water mobile phase. Study on the band broadening effect of holding a solute in a column or elution at very low linear velocity of mobile...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of Chromatography A 2006-03, Vol.1109 (1), p.2-9
Hauptverfasser:	Kobayashi, Hiroshi, Tokuda, Daisuke, Ichimaru, Jun, Ikegami, Tohru, Miyabe, Kanji, Tanaka, Nobuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Band dispersion Benzene Derivatives - chemistry Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Diffusion Molecular diffusion Monolithic silica column Organic Chemicals - isolation & purification Silicon Dioxide Uracil - isolation & purification
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The contribution of molecular diffusion to peak broadening was studied in a reversed-phase HPLC system, consisting of a monolithic silica C 18 column and methanol–water mobile phase. Study on the band broadening effect of holding a solute in a column or elution at very low linear velocity of mobile phase allowed facile determination of the contribution of the molecular diffusion term. Less obstruction against molecular diffusion, or the faster axial band dispersion in a monolithic silica column than in a particle-packed column, was found both in mobile phase and in stationary phase.
ISSN:	0021-9673
DOI:	10.1016/j.chroma.2005.11.053