New antibacterial peptide derived from bovine hemoglobin

Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields an intermediate peptide fraction exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli and Salmonella enteritidis after separation by reversed-phase HPLC. From this fraction a pur...

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Veröffentlicht in:	Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2005-05, Vol.26 (5), p.713-719
Hauptverfasser:	Daoud, Rachid, Dubois, Veronique, Bors-Dodita, Loredana, Nedjar-Arroume, Naima, Krier, Francois, Chihib, Nour-Eddine, Mary, Patrice, Kouach, Mostafa, Briand, Gilbert, Guillochon, Didier
Format:	Artikel
Sprache:	eng
Schlagworte:	Active peptide Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibacterial peptide Biological and medical sciences Bovine hemoglobin Cattle Escherichia coli Escherichia coli - drug effects Fundamental and applied biological sciences. Psychology Hemoglobins - chemistry Hemoglobins - pharmacology Hemolysis Hydrolysate Hydrolysis Listeria - drug effects Listeria innocua Micrococcus luteus Micrococcus luteus - drug effects Pepsin Peptide Fragments - chemistry Peptide Fragments - pharmacology Peptides - chemistry Peptides - pharmacology Salmonella enteritidis Salmonella enteritidis - drug effects Vertebrates: endocrinology
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Zusammenfassung:	Peptic digestion of bovine hemoglobin at low degree of hydrolysis yields an intermediate peptide fraction exhibiting antibacterial activity against Micrococcus luteus A270, Listeria innocua, Escherichia coli and Salmonella enteritidis after separation by reversed-phase HPLC. From this fraction a pure peptide was isolated and analyzed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS). This peptide correspond to the 107–136 fragment of the α chain of bovine hemoglobin. The minimum inhibitory concentrations (MIC) towards the four strains and its hemolytic activity towards bovine erythrocytes were determined. A MIC of 38 μM was reported against L. innocua and 76 μM for other various bacterial species. This peptide had no hemolytic activity up to 380 μM concentration.
ISSN:	0196-9781 1873-5169
DOI:	10.1016/j.peptides.2004.12.008