Leptospira broomii sp. nov., isolated from humans with leptospirosis

Meningitis and Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA Correspondence Paul N. Levett plevett{at}health.gov.sk.ca Isolates of Leptospira from two human cases of leptospirosis in Denmark and France were studied using DNA–DNA relatedness, G+C content, 16S rRNA gene sequence data and pulsed-field gel electrophoresis. These isolates differed from previously described species of Leptospira and are defined as Leptospira broomii sp. nov. The type strain is 5399 T (=ATCC BAA-1107 T =KIT 5399 T ). Abbreviations: PFGE, pulsed-field gel electrophoresis Published online ahead of print on 2 December 2005 as DOI 10.1099/ijs.0.63783-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Leptospira broomii strains 5399 T and L065 are AY796065 and AY792329 . Present address: Saskatchewan Health, Provincial Laboratory, 3211 Albert Street, Regina, Saskatchewan S4S 5W6, Canada.