Use of housekeeping gene sequencing for species identification of viridans streptococci

Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis–sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. Th...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Diagnostic microbiology and infectious disease 2005-04, Vol.51 (4), p.297-301
Hauptverfasser:	Kiratisin, Pattarachai, Li, Li, Murray, Patrick R., Fischer, Steven H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Typing Techniques Biological and medical sciences DNA, Bacterial - analysis Fundamental and applied biological sciences. Psychology gki Glucokinase - genetics Glucosephosphate Dehydrogenase - genetics Housekeeping genes Humans Infectious diseases Medical sciences Microbiology Sequence Analysis, DNA Species Specificity Streptococcus Viridans Streptococci - classification Viridans Streptococci - genetics zwf
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Based on the genetic analysis of 20 reference strains, we describe an approach using the sequencing of 2 housekeeping genes, zwf and gki, to identify members of the mitis–sanguinis group of viridans streptococci to the species level, with a better discrimination compared with 16S rDNA sequencing. This approach also suggested that some reference strains may not be correctly classified.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2004.12.001