Enzymatic Incorporation of an Antibody-Activated Blue Fluorophore into DNA

Antibodies as light switches: The deoxynucleotide analogue–stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene‐modified nucleotide. Therefore, DN...

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Veröffentlicht in:	Angewandte Chemie International Edition 2005-03, Vol.44 (14), p.2144-2148
Hauptverfasser:	Kaufmann, Gunnar F., Meijler, Michael M., Sun, Chengzao, Chen, Da-Wei, Kujawa, David P., Mee, Jenny M., Hoffman, Timothy Z., Wirsching, Peter, Lerner, Richard A., Janda, Kim D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analogs & derivatives Adenosine Triphosphate - chemical synthesis Adenosine Triphosphate - chemistry antibodies Antibodies - chemistry DNA - chemistry DNA Probes - chemical synthesis DNA Probes - chemistry DNA recognition Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry fluorescent probes Molecular Structure nucleotides polymerase chain reaction Stilbenes - chemistry
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Beschreibung
Zusammenfassung:	Antibodies as light switches: The deoxynucleotide analogue–stilbene conjugate 1 can be incorporated into nascent DNA by DNA polymerase activity. The blue fluorescence of stilbene is detected only upon binding of an antibody that specifically recognizes the stilbene‐modified nucleotide. Therefore, DNA generated through PCR with 1 in the substrate pool can be used for hybridization assays in which detection is triggered by the antibody (see scheme).
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.200461143