Yeast two-hybrid detection systems that are highly sensitive to a certain kind of endocrine disruptors
We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1)...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-02, Vol.70 (2), p.521-524 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Lee, H.S.(Tokyo Univ. (Japan)) Sasagawa, S Kato, S Fukuda, R Horiuchi, H Ohta, A |
| description | We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen dependent induction of the chromosome-integrated UASsub(GAL)-CYC1sup(P)-lacZ reporter construct among the two-hybrid systems so far tested. |
| doi_str_mv | 10.1271/bbb.70.521 |
| format | Article |
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A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen dependent induction of the chromosome-integrated UASsub(GAL)-CYC1sup(P)-lacZ reporter construct among the two-hybrid systems so far tested.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.70.521</identifier><identifier>PMID: 16495672</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Biological and medical sciences ; CONTROL HORMONAL ; CONTROLE HORMONAL ; endocrine disruptor ; Endocrine disruptors ; ESTEROIDES ; estrogen ; Estrogen Receptor alpha - genetics ; Estrogen Receptor alpha - metabolism ; ESTROGENOS ; Estrogens - pharmacology ; Fundamental and applied biological sciences. Psychology ; Genes, Reporter - genetics ; Histone Acetyltransferases ; HORMONAL CONTROL ; HORMONE RECEPTORS ; human estrogen receptor-α (hERα) ; Humans ; METHODE D'ESSAI ; METODOS DE ENSAYO ; Nuclear Receptor Coactivator 1 ; Nuclear Receptor Coactivator 2 - genetics ; Nuclear Receptor Coactivator 2 - metabolism ; OESTROGENE ; OESTROGENS ; Protein Binding - drug effects ; RECEPTEUR D'HORMONE ; RECEPTORES DE HORMONAS ; Sensitivity and Specificity ; steroid receptor co-activator-1 (SRC-1) ; STEROIDE ; STEROIDS ; Transcription Factors - genetics ; Transcription Factors - metabolism ; TRIAL METHODS ; Two-Hybrid System Techniques ; yeast two-hybrid system</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2006-02, Vol.70 (2), p.521-524</ispartof><rights>2006 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2006</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c568t-a72c4c520cd0cd1cf7008590ea8adfa4cea5770816f406f040de5133984437f83</citedby><cites>FETCH-LOGICAL-c568t-a72c4c520cd0cd1cf7008590ea8adfa4cea5770816f406f040de5133984437f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17703835$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16495672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, H.S.(Tokyo Univ. (Japan))</creatorcontrib><creatorcontrib>Sasagawa, S</creatorcontrib><creatorcontrib>Kato, S</creatorcontrib><creatorcontrib>Fukuda, R</creatorcontrib><creatorcontrib>Horiuchi, H</creatorcontrib><creatorcontrib>Ohta, A</creatorcontrib><title>Yeast two-hybrid detection systems that are highly sensitive to a certain kind of endocrine disruptors</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen dependent induction of the chromosome-integrated UASsub(GAL)-CYC1sup(P)-lacZ reporter construct among the two-hybrid systems so far tested.</description><subject>Biological and medical sciences</subject><subject>CONTROL HORMONAL</subject><subject>CONTROLE HORMONAL</subject><subject>endocrine disruptor</subject><subject>Endocrine disruptors</subject><subject>ESTEROIDES</subject><subject>estrogen</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>ESTROGENOS</subject><subject>Estrogens - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Reporter - genetics</subject><subject>Histone Acetyltransferases</subject><subject>HORMONAL CONTROL</subject><subject>HORMONE RECEPTORS</subject><subject>human estrogen receptor-α (hERα)</subject><subject>Humans</subject><subject>METHODE D'ESSAI</subject><subject>METODOS DE ENSAYO</subject><subject>Nuclear Receptor Coactivator 1</subject><subject>Nuclear Receptor Coactivator 2 - genetics</subject><subject>Nuclear Receptor Coactivator 2 - metabolism</subject><subject>OESTROGENE</subject><subject>OESTROGENS</subject><subject>Protein Binding - drug effects</subject><subject>RECEPTEUR D'HORMONE</subject><subject>RECEPTORES DE HORMONAS</subject><subject>Sensitivity and Specificity</subject><subject>steroid receptor co-activator-1 (SRC-1)</subject><subject>STEROIDE</subject><subject>STEROIDS</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>TRIAL METHODS</subject><subject>Two-Hybrid System Techniques</subject><subject>yeast two-hybrid system</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctrFTEUB-Agir2tbtwrAakLYa7J5DVZSvFJQRe6cBUyefSmziS3ScYy_70p90pBBCEQCN85Sc4PgGcYbXEv8JtxHLcCbVmPH4ANJlR0XFLxEGyQxLwbKMMn4LSUa4TaAcOPwQnmVDIu-g3wP5wuFdbb1O3WMQcLravO1JAiLGupbi6w7nSFOju4C1e7aYXFxRJq-OVgTVBD43LVIcKfIVqYPHTRJpNDdNCGkpd9Tbk8AY-8nop7etzPwPf3775dfOwuv3z4dPH2sjOMD7XTojfUsB4Z2xY2XiA0MImcHrT1mhqnmRBowNxTxD2iyDqGCZEDpUT4gZyBV4e--5xuFleqmkMxbpp0dGkpigsuESPyvxBL2nNCUIMv_4LXacmxfUJhSuWAaS_u7n19UCanUrLzap_DrPOqMFJ3IakWkhJItZAafnFsuYyzs_f0mEoD50egi9GTzzqaUO5dGwEZCGuOHVyIPuVZ36Y8WVX1OqX8p4j88wHPD3VeJ6WvcmOfv_YItWkTKin5Dad6tL0</recordid><startdate>20060201</startdate><enddate>20060201</enddate><creator>Lee, H.S.(Tokyo Univ. 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Psychology</topic><topic>Genes, Reporter - genetics</topic><topic>Histone Acetyltransferases</topic><topic>HORMONAL CONTROL</topic><topic>HORMONE RECEPTORS</topic><topic>human estrogen receptor-α (hERα)</topic><topic>Humans</topic><topic>METHODE D'ESSAI</topic><topic>METODOS DE ENSAYO</topic><topic>Nuclear Receptor Coactivator 1</topic><topic>Nuclear Receptor Coactivator 2 - genetics</topic><topic>Nuclear Receptor Coactivator 2 - metabolism</topic><topic>OESTROGENE</topic><topic>OESTROGENS</topic><topic>Protein Binding - drug effects</topic><topic>RECEPTEUR D'HORMONE</topic><topic>RECEPTORES DE HORMONAS</topic><topic>Sensitivity and Specificity</topic><topic>steroid receptor co-activator-1 (SRC-1)</topic><topic>STEROIDE</topic><topic>STEROIDS</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>TRIAL METHODS</topic><topic>Two-Hybrid System Techniques</topic><topic>yeast two-hybrid system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, H.S.(Tokyo Univ. 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A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen dependent induction of the chromosome-integrated UASsub(GAL)-CYC1sup(P)-lacZ reporter construct among the two-hybrid systems so far tested.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>16495672</pmid><doi>10.1271/bbb.70.521</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2006-02, Vol.70 (2), p.521-524 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67690539 |
| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Biological and medical sciences CONTROL HORMONAL CONTROLE HORMONAL endocrine disruptor Endocrine disruptors ESTEROIDES estrogen Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism ESTROGENOS Estrogens - pharmacology Fundamental and applied biological sciences. Psychology Genes, Reporter - genetics Histone Acetyltransferases HORMONAL CONTROL HORMONE RECEPTORS human estrogen receptor-α (hERα) Humans METHODE D'ESSAI METODOS DE ENSAYO Nuclear Receptor Coactivator 1 Nuclear Receptor Coactivator 2 - genetics Nuclear Receptor Coactivator 2 - metabolism OESTROGENE OESTROGENS Protein Binding - drug effects RECEPTEUR D'HORMONE RECEPTORES DE HORMONAS Sensitivity and Specificity steroid receptor co-activator-1 (SRC-1) STEROIDE STEROIDS Transcription Factors - genetics Transcription Factors - metabolism TRIAL METHODS Two-Hybrid System Techniques yeast two-hybrid system |
| title | Yeast two-hybrid detection systems that are highly sensitive to a certain kind of endocrine disruptors |
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