Yeast two-hybrid detection systems that are highly sensitive to a certain kind of endocrine disruptors

We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1)...

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Veröffentlicht in:	Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-02, Vol.70 (2), p.521-524
Hauptverfasser:	Lee, H.S.(Tokyo Univ. (Japan)), Sasagawa, S, Kato, S, Fukuda, R, Horiuchi, H, Ohta, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences CONTROL HORMONAL CONTROLE HORMONAL endocrine disruptor Endocrine disruptors ESTEROIDES estrogen Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism ESTROGENOS Estrogens - pharmacology Fundamental and applied biological sciences. Psychology Genes, Reporter - genetics Histone Acetyltransferases HORMONAL CONTROL HORMONE RECEPTORS human estrogen receptor-α (hERα) Humans METHODE D'ESSAI METODOS DE ENSAYO Nuclear Receptor Coactivator 1 Nuclear Receptor Coactivator 2 - genetics Nuclear Receptor Coactivator 2 - metabolism OESTROGENE OESTROGENS Protein Binding - drug effects RECEPTEUR D'HORMONE RECEPTORES DE HORMONAS Sensitivity and Specificity steroid receptor co-activator-1 (SRC-1) STEROIDE STEROIDS Transcription Factors - genetics Transcription Factors - metabolism TRIAL METHODS Two-Hybrid System Techniques yeast two-hybrid system
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Zusammenfassung:	We tested the effects of several combinations of bait and fish components of the yeast two-hybrid detection system for estrogenic activity. A combination of the full-length human estrogen receptor alpha with the nuclear receptor-binding domain of co-activator steroid receptor co-activator-1 (SRC-1) or transcriptional intermediate factor-2 (TIF-2) was most effective for estrogen dependent induction of the chromosome-integrated UASsub(GAL)-CYC1sup(P)-lacZ reporter construct among the two-hybrid systems so far tested.
ISSN:	0916-8451 1347-6947
DOI:	10.1271/bbb.70.521