Purification and Kinase Assay of PKN

Purification and Kinase Assay of PKN

PKN is a serine/threonine protein kinase, which has a catalytic domain highly homologous to that of protein kinase C (PKC) in the carboxyl‐terminal region and three repeats of the antiparallel coiled coil (ACC) domain in the amino‐terminal region. Mammalian PKN has three isoforms each derived from d...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Mukai, Hideyuki, Ono, Yoshitaka
Format: Buchkapitel
Sprache:eng
Schlagworte:
Animals
Brain - enzymology
Cattle
Cytosol - enzymology
Enzyme Activation
Humans
Isoenzymes - metabolism
Male
Molecular biology
Protein Kinase C
Protein-Serine-Threonine Kinases - isolation & purification
Protein-Serine-Threonine Kinases - metabolism
Protein-Tyrosine Kinases - isolation & purification
Protein-Tyrosine Kinases - metabolism
Proteins
Rats
Recombinant Proteins - isolation & purification
Spodoptera
Testis - enzymology
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Beschreibung
Zusammenfassung:PKN is a serine/threonine protein kinase, which has a catalytic domain highly homologous to that of protein kinase C (PKC) in the carboxyl‐terminal region and three repeats of the antiparallel coiled coil (ACC) domain in the amino‐terminal region. Mammalian PKN has three isoforms each derived from different genes, PKN1 (PKNα/PRK1/PAK1), PKN2 (PRK2/PAK2/PKNγ), and PKN3 (PKNβ). PKN isoforms show different enzymatic properties and tissue distributions and have been implicated in various distinct cellular processes (reviewed in Mukai [2003]). This chapter discusses methods to prepare purified enzymes and to assay substrate phosphorylation activities.
ISSN:0076-6879
1557-7988
DOI:10.1016/S0076-6879(06)06017-4