The recombination deficient Enterococcus faecalis UV202 strain is a recA mutant

The recA gene of the recombination deficient Enterococcus faecalis strain UV202 was sequenced and found to encode a glycine to aspartic acid mutation at amino acid 265. Both the UV sensitive and recombination deficient phenotypes of the UV202 strain were complemented by expression of the wild-type recA gene cloned under the control of the nisin-inducible promoter of an expression vector.