Fodinibacter luteus gen. nov., sp. nov., an actinobacterium isolated from a salt mine

1 Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, PR China 2 College of Bio-resources and Environmental Science, Jishou University, Jishou, Hunan 416000, PR China 3 Agricultural Cultural Collection of China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100080, PR China Correspondence Xiao-Long Cui xlcui{at}ynu.edu.cn A Gram-positive-staining, aerobic, catalase- and oxidase-positive, irregular short rod-shaped actinobacterium, designated strain YIM C003 T , was isolated from a salt mine in Yunnan, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM C003 T was most closely related to strains of the genera Knoellia (94.2–96.0 % similarity), Oryzihumus (95.6 %), Terrabacter (94.9–95.4 %), Janibacter (94.9–95.4 %), Kribbia (95.0 %), Lapillicoccus (95.0 %) and Phycicoccus (94.2–95.0 %) of the family Intrasporangiaceae and that it formed an independent monophyletic lineage with three strains of Oryzihumus leptocrescens . The DNA G+C content of strain YIM C003 T was 72.0 mol%. The diagnostic cell-wall diamino acid was meso -diaminopimelic acid. The predominant menaquinone was MK-8(H 4 ). Mycolic acids were not detected. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids. The major cellular fatty acids were C 18 : 1 9 c and C 16 : 0 . These chemotaxonomic properties, together with data from phylogenetic analysis, enabled the novel isolate to be differentiated from all other members of the family. A novel species in a new genus, Fodinibacter luteus gen. nov., sp. nov., is proposed, with strain YIM C003 T (=DSM 21208 T =CCTCC AA 208036 T ) as the type strain of Fodinibacter luteus . Abbreviations: DAP, diaminopimelic acid These authors contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM C003 T is EU878005 . A transmission electron micrograph of cells of strain YIM C003 T is available as supplementary material with the online version of this paper.