Transcription promotes contraction of CAG repeat tracts in human cells

Transcription promotes contraction of CAG repeat tracts in human cells

Induced transcription through CAG repeats in human cells increases repeat contraction ∼15-fold in both confluent and proliferating cells. Repeats are stabilized against contraction by siRNA knockdown of MSH2, MSH3 or XPA, but not by knockdown of MSH6, XPC or FEN1. These results define a pathway for...

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Veröffentlicht in:Nature structural & molecular biology 2006-02, Vol.13 (2), p.179-180
Hauptverfasser: Lin, Yunfu, Dion, Vincent, Wilson, John H
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Biochemistry
Biological Microscopy
Biomedical and Life Sciences
brief-communication
Cell Line, Tumor
Cell proliferation
Deoxyribonucleic acid
DNA
DNA binding proteins
DNA repair
Genetic transcription
Humans
Life Sciences
Membrane Biology
Molecular biology
Physiological aspects
Protein Structure
RNA, Small Interfering - genetics
Sequence Deletion - genetics
Structure
Transcription, Genetic - genetics
Trinucleotide Repeats - genetics
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Zusammenfassung:Induced transcription through CAG repeats in human cells increases repeat contraction ∼15-fold in both confluent and proliferating cells. Repeats are stabilized against contraction by siRNA knockdown of MSH2, MSH3 or XPA, but not by knockdown of MSH6, XPC or FEN1. These results define a pathway for CAG·CTG repeat contraction that is initiated by transcription, depends on elements of mismatch and nucleotide-excision repair and does not require DNA replication.
ISSN:1545-9993
1545-9985
DOI:10.1038/nsmb1042