Secondary metabolites from Ganoderma lucidum and Spongiporus leucomallellus

Ganoderma lucidum contains the steroidester ergosta-7,22-diene-3β-yl pentadecanoate ( 3) while Spongiporus leucomallellus contains the two triterpene acids named spongiporic acids A ( 11) and B ( 12). Antimicrobial activities of spongiporic acid A and cytotoxic activities of 5,8- epi-dioxy-24-methylcholesta-6,9(11),22-triene-3β-ol were investigated. The hydrodistillates and solvent extracts of the fruit bodies of Ganoderma lucidum (Fr.) P. Karst. and Spongiporus leucomallellus (Murril) A. David were investigated. The constituents in both oils comprised hydrocarbons, monoterpenes, sesquiterpenes, and fatty acids. Major volatiles of G. lucidum were trans-anethol, R-(−)-linalool, S-(+)-carvone and α-bisabolol, while the essential oil of S. leucomallellus contained relatively large amounts of R-(−)-1-octene-3-ol, R-(−)-linalool, 1-hepten-3-one and ( Z)-nerolidol. From the n-hexane extract of G. lucidum, the steroid ester ergosta-7,22-diene-3β-yl pentadecanoate could be identified. From S. leucomallellus two constituents showing structures of 3,4- seco-lanostane type triterpene acids were identified as (+)-23-oxo-3,4- seco-lanosta-4(28),7(8),9(11),24(31)-tetraene-3,26-dicarboxylic acid and (+)-20-hydroxy-23-oxo-3,4- seco-lanosta-4(28),7(8),9(11),24(31)-tetraene3,26-dicarboxylic acid, respectively. Cytotoxicity and antimicrobial activity of selected compounds were investigated using standard tests.