Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re

A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol–water–acetic acid (45:55:1, by...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-01, Vol.830 (1), p.100-104
Hauptverfasser:	Morinaga, Osamu, Tanaka, Hiroyuki, Shoyama, Yukihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Analytical, structural and metabolic biochemistry Animals Antibodies, Monoclonal - immunology Biological and medical sciences Chromatographic immunostaining Chromatography, Thin Layer - methods Female Fundamental and applied biological sciences. Psychology General pharmacology Ginseng Ginsenoside Re Ginsenosides - analysis Ginsenosides - immunology Medical sciences Mice Mice, Inbred BALB C NIH Image software Nucleic Acid Hybridization Panax - chemistry Pharmacology. Drug treatments Reproducibility of Results
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Zusammenfassung:	A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol–water–acetic acid (45:55:1, by volume). G-Re was clearly detected by an immunostaining method using a monoclonal antibody against G-Re. The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 μg of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 4.0 μg.
ISSN:	1570-0232 1873-376X
DOI:	10.1016/j.jchromb.2005.10.040