Fluorescent sensors for rapid monitoring of intracellular cGMP

Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phospho...

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Veröffentlicht in:	Nature methods 2006-01, Vol.3 (1), p.23-25
Hauptverfasser:	Nikolaev, Viacheslav O, Gambaryan, Stepan, Lohse, Martin J
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biosensing Techniques brief-communication Cells, Cultured Cyclic AMP - analysis Cyclic GMP - analysis Cyclic GMP-Dependent Protein Kinase Type I Cyclic GMP-Dependent Protein Kinases - chemistry Cyclic GMP-Dependent Protein Kinases - genetics Energy transfer Fluorescence Fluorescence Resonance Energy Transfer Humans Life Sciences Mammals Phosphoric Diester Hydrolases - chemistry Phosphoric Diester Hydrolases - genetics Protein Structure, Tertiary - genetics Proteomics Resonance Sensors Sequence Deletion
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Beschreibung
Zusammenfassung:	Sensors based on fluorescence resonance energy transfer (FRET) are powerful tools to monitor signaling events in living mammalian cells. Here we describe development and use of new sensors for cyclic GMP (cGMP) based on cGMP binding domains from cGMP-dependent protein kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and spatial resolution attained with the new sensors is superior to that of existing techniques, and permits direct recording and imaging of rapid cGMP-signaling events.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth816