Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical systems and species-specific PCR

One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK®2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed...

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Veröffentlicht in:	Journal of microbiological methods 2006, Vol.64 (1), p.135-138
Hauptverfasser:	Giordano, A., Magni, A., Trancassini, M., Varesi, P., Turner, R., Mancini, C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological methods and techniques used in bacteriology Bacteriological Techniques - methods Bacteriology Biochemical Biological and medical sciences Bronchi - microbiology Cystic Fibrosis - microbiology Fundamental and applied biological sciences. Psychology Gram-Negative Bacterial Infections - epidemiology Gram-Negative Bacterial Infections - microbiology Humans Identification Microbiology Molecular Polymerase Chain Reaction - methods Species Specificity Sputum - microbiology Stenotrophomonas maltophilia Stenotrophomonas maltophilia - classification Stenotrophomonas maltophilia - genetics Stenotrophomonas maltophilia - isolation & purification Trachea - microbiology
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Zusammenfassung:	One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK®2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed only 1 strain with “low discrimination” whereas the VITEK®2 gave 12. In any case, the two biochemical systems showed good reliability compared to SS-PCR.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2005.04.020