A fish antimicrobial peptide, tilapia hepcidin TH2-3, shows potent antitumor activity against human fibrosarcoma cells
As part of a continuing search for potential anticancer drug candidates from antimicrobial peptides of marine organisms, tilapia ( Oreochromis mossambicus) hepcidin TH2-3 was evaluated in several tumor cell lines. The results indicated that TH2-3, a synthetic 20-mer antimicrobial peptide, specifical...
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Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2009-09, Vol.30 (9), p.1636-1642 |
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Sprache: | eng |
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Zusammenfassung: | As part of a continuing search for potential anticancer drug candidates from antimicrobial peptides of marine organisms, tilapia (
Oreochromis mossambicus) hepcidin TH2-3 was evaluated in several tumor cell lines. The results indicated that TH2-3, a synthetic 20-mer antimicrobial peptide, specifically inhibited human fibrosarcoma cell (HT1080 cell line) proliferation and migration. The way in which TH2-3 inhibited HT1080 cell growth was then studied. TH2-3 inhibited HT1080 cell growth in a concentration-dependent manner according to an MTT analysis, which was confirmed by a soft-agar assay and AO/EtBr staining. Scanning electron microscopy revealed that TH2-3 caused lethal membrane disruption in HT1080 cancer cells, and a wound healing assay supported that TH2-3 decreased the migration of HT1080 cells. In addition, c-Jun mRNA expression was downregulated after treatment with TH2-3 for 48–96
h compared to the untreated group. These findings suggest a mechanism of cytotoxic action of TH2-3 and indicate that TH2-3 may be a promising chemotherapeutic agent against human fibrosarcoma cells. |
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ISSN: | 0196-9781 1873-5169 |
DOI: | 10.1016/j.peptides.2009.06.009 |