Isolation of Murine and Porcine Fetal Stem Cells from Somatic Tissue
Adult stem cells have been previously isolated from a variety of somatic tissues, including bone marrow and the central nervous system; however, contribution of these cells to the germ line has not been shown. Here we demonstrate that fetal somatic explants contain a subpopulation of somatic stem ce...
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| Veröffentlicht in: | Biology of reproduction 2005-04, Vol.72 (4), p.1020-1028 |
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| container_title | Biology of reproduction |
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| creator | KUES, Wilfried A PETERSEN, Björn MYSEGADES, Wiebke CARNWATH, Joseph W NIEMANN, Heiner |
| description | Adult stem cells have been previously isolated from a variety of somatic tissues, including bone marrow and the central nervous
system; however, contribution of these cells to the germ line has not been shown. Here we demonstrate that fetal somatic explants
contain a subpopulation of somatic stem cells (FSSCs), which can be induced to display features of lineage-uncommitted stem
cells. After injection into blastocysts, these cells give rise to a variety of cell types in the resultant chimeric fetuses,
including those of the mesodermal lineage; they even migrate into the genital ridge. In vitro, FSSCs exhibit characteristics
of embryonic stem cells, including extended self-renewal; expression of stem cell marker genes, such as Pou5f1 ( Oct4 ), Stat3 , and Akp2 ( Tnap ) and growth as multicellular aggregates. We report that fetal tissue contains somatic stem cells with greater potency than
previously thought, which might form a new source of stem cells useful in somatic nuclear transfer and cell therapy.
Abstract
Fetal tissue contains somatic stem cells with greater potency than previously thought, which might form a new source of stem
cells useful in somatic nuclear transfer and cell therapy |
| doi_str_mv | 10.1095/biolreprod.104.031229 |
| format | Article |
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system; however, contribution of these cells to the germ line has not been shown. Here we demonstrate that fetal somatic explants
contain a subpopulation of somatic stem cells (FSSCs), which can be induced to display features of lineage-uncommitted stem
cells. After injection into blastocysts, these cells give rise to a variety of cell types in the resultant chimeric fetuses,
including those of the mesodermal lineage; they even migrate into the genital ridge. In vitro, FSSCs exhibit characteristics
of embryonic stem cells, including extended self-renewal; expression of stem cell marker genes, such as Pou5f1 ( Oct4 ), Stat3 , and Akp2 ( Tnap ) and growth as multicellular aggregates. We report that fetal tissue contains somatic stem cells with greater potency than
previously thought, which might form a new source of stem cells useful in somatic nuclear transfer and cell therapy.
Abstract
Fetal tissue contains somatic stem cells with greater potency than previously thought, which might form a new source of stem
cells useful in somatic nuclear transfer and cell therapy</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.104.031229</identifier><identifier>PMID: 15616223</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Blastomeres - cytology ; Cell Adhesion ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell Division ; Cell Lineage ; Cell Separation - methods ; Cells, Cultured ; Chimera ; Connective Tissue - embryology ; DNA-Binding Proteins - genetics ; Female ; Fetus - cytology ; Fibroblasts - cytology ; Fundamental and applied biological sciences. Psychology ; Green Fluorescent Proteins - genetics ; Mammalian female genital system ; Mice ; Mice, Transgenic ; Morphology. Physiology ; Octamer Transcription Factor-3 ; Pluripotent Stem Cells - cytology ; Pregnancy ; Promoter Regions, Genetic - physiology ; RNA, Messenger - analysis ; Spheroids, Cellular ; Swine ; Transcription Factors - genetics ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2005-04, Vol.72 (4), p.1020-1028</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16633647$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15616223$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KUES, Wilfried A</creatorcontrib><creatorcontrib>PETERSEN, Björn</creatorcontrib><creatorcontrib>MYSEGADES, Wiebke</creatorcontrib><creatorcontrib>CARNWATH, Joseph W</creatorcontrib><creatorcontrib>NIEMANN, Heiner</creatorcontrib><title>Isolation of Murine and Porcine Fetal Stem Cells from Somatic Tissue</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Adult stem cells have been previously isolated from a variety of somatic tissues, including bone marrow and the central nervous
system; however, contribution of these cells to the germ line has not been shown. Here we demonstrate that fetal somatic explants
contain a subpopulation of somatic stem cells (FSSCs), which can be induced to display features of lineage-uncommitted stem
cells. After injection into blastocysts, these cells give rise to a variety of cell types in the resultant chimeric fetuses,
including those of the mesodermal lineage; they even migrate into the genital ridge. In vitro, FSSCs exhibit characteristics
of embryonic stem cells, including extended self-renewal; expression of stem cell marker genes, such as Pou5f1 ( Oct4 ), Stat3 , and Akp2 ( Tnap ) and growth as multicellular aggregates. We report that fetal tissue contains somatic stem cells with greater potency than
previously thought, which might form a new source of stem cells useful in somatic nuclear transfer and cell therapy.
Abstract
Fetal tissue contains somatic stem cells with greater potency than previously thought, which might form a new source of stem
cells useful in somatic nuclear transfer and cell therapy</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastomeres - cytology</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>Cell Lineage</subject><subject>Cell Separation - methods</subject><subject>Cells, Cultured</subject><subject>Chimera</subject><subject>Connective Tissue - embryology</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Female</subject><subject>Fetus - cytology</subject><subject>Fibroblasts - cytology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Mammalian female genital system</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Morphology. Physiology</subject><subject>Octamer Transcription Factor-3</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pregnancy</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>RNA, Messenger - analysis</subject><subject>Spheroids, Cellular</subject><subject>Swine</subject><subject>Transcription Factors - genetics</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKBDEQRYMoOj4-QclGd61JKo_OUsYnKArqukn3JE4k3dGkm8G_N-KIS1dVBadu3VsIHVJySokWZ62PIdn3FBdl5qcEKGN6A82oYLpSTNabaEYIkRWAhB20m_MbIZQDg220Q4WkkjGYoYvbHIMZfRxwdPh-Sn6w2AwL_BhT991f2dEE_DTaHs9tCBm7FHv8FPuy1OFnn_Nk99GWMyHbg3XdQy9Xl8_zm-ru4fp2fn5XLYHQsZJKuoWyoDQntSIUhLMggNctUVw727ZGcF28t64kpNxxKRx0IGrlGLMO9tDJj26J_THZPDa9z11xZQYbp9xIJYBqxv8FqdayZgoKeLQGp7a3i-Y9-d6kz-b3QQU4XgMmdya4ZIbO5z9OyvJfrv4uLv3rcuWTbXJvQiiy0KxWK8Ua3lDCCHwBzjGCVQ</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>KUES, Wilfried A</creator><creator>PETERSEN, Björn</creator><creator>MYSEGADES, Wiebke</creator><creator>CARNWATH, Joseph W</creator><creator>NIEMANN, Heiner</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050401</creationdate><title>Isolation of Murine and Porcine Fetal Stem Cells from Somatic Tissue</title><author>KUES, Wilfried A ; PETERSEN, Björn ; MYSEGADES, Wiebke ; CARNWATH, Joseph W ; NIEMANN, Heiner</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h301t-676fd7e37940870135fe35348b0749febba549726bf09514f465f3c3587f22ef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastomeres - cytology</topic><topic>Cell Adhesion</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>Cell Lineage</topic><topic>Cell Separation - methods</topic><topic>Cells, Cultured</topic><topic>Chimera</topic><topic>Connective Tissue - embryology</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Female</topic><topic>Fetus - cytology</topic><topic>Fibroblasts - cytology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Mammalian female genital system</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Morphology. Physiology</topic><topic>Octamer Transcription Factor-3</topic><topic>Pluripotent Stem Cells - cytology</topic><topic>Pregnancy</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>RNA, Messenger - analysis</topic><topic>Spheroids, Cellular</topic><topic>Swine</topic><topic>Transcription Factors - genetics</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KUES, Wilfried A</creatorcontrib><creatorcontrib>PETERSEN, Björn</creatorcontrib><creatorcontrib>MYSEGADES, Wiebke</creatorcontrib><creatorcontrib>CARNWATH, Joseph W</creatorcontrib><creatorcontrib>NIEMANN, Heiner</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KUES, Wilfried A</au><au>PETERSEN, Björn</au><au>MYSEGADES, Wiebke</au><au>CARNWATH, Joseph W</au><au>NIEMANN, Heiner</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Murine and Porcine Fetal Stem Cells from Somatic Tissue</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2005-04-01</date><risdate>2005</risdate><volume>72</volume><issue>4</issue><spage>1020</spage><epage>1028</epage><pages>1020-1028</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Adult stem cells have been previously isolated from a variety of somatic tissues, including bone marrow and the central nervous
system; however, contribution of these cells to the germ line has not been shown. Here we demonstrate that fetal somatic explants
contain a subpopulation of somatic stem cells (FSSCs), which can be induced to display features of lineage-uncommitted stem
cells. After injection into blastocysts, these cells give rise to a variety of cell types in the resultant chimeric fetuses,
including those of the mesodermal lineage; they even migrate into the genital ridge. In vitro, FSSCs exhibit characteristics
of embryonic stem cells, including extended self-renewal; expression of stem cell marker genes, such as Pou5f1 ( Oct4 ), Stat3 , and Akp2 ( Tnap ) and growth as multicellular aggregates. We report that fetal tissue contains somatic stem cells with greater potency than
previously thought, which might form a new source of stem cells useful in somatic nuclear transfer and cell therapy.
Abstract
Fetal tissue contains somatic stem cells with greater potency than previously thought, which might form a new source of stem
cells useful in somatic nuclear transfer and cell therapy</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>15616223</pmid><doi>10.1095/biolreprod.104.031229</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biology of reproduction, 2005-04, Vol.72 (4), p.1020-1028 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Blastomeres - cytology Cell Adhesion Cell Culture Techniques - methods Cell Differentiation Cell Division Cell Lineage Cell Separation - methods Cells, Cultured Chimera Connective Tissue - embryology DNA-Binding Proteins - genetics Female Fetus - cytology Fibroblasts - cytology Fundamental and applied biological sciences. Psychology Green Fluorescent Proteins - genetics Mammalian female genital system Mice Mice, Transgenic Morphology. Physiology Octamer Transcription Factor-3 Pluripotent Stem Cells - cytology Pregnancy Promoter Regions, Genetic - physiology RNA, Messenger - analysis Spheroids, Cellular Swine Transcription Factors - genetics Vertebrates: reproduction |
| title | Isolation of Murine and Porcine Fetal Stem Cells from Somatic Tissue |
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