Isolation of Murine and Porcine Fetal Stem Cells from Somatic Tissue
Adult stem cells have been previously isolated from a variety of somatic tissues, including bone marrow and the central nervous system; however, contribution of these cells to the germ line has not been shown. Here we demonstrate that fetal somatic explants contain a subpopulation of somatic stem ce...
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Veröffentlicht in: | Biology of reproduction 2005-04, Vol.72 (4), p.1020-1028 |
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Zusammenfassung: | Adult stem cells have been previously isolated from a variety of somatic tissues, including bone marrow and the central nervous
system; however, contribution of these cells to the germ line has not been shown. Here we demonstrate that fetal somatic explants
contain a subpopulation of somatic stem cells (FSSCs), which can be induced to display features of lineage-uncommitted stem
cells. After injection into blastocysts, these cells give rise to a variety of cell types in the resultant chimeric fetuses,
including those of the mesodermal lineage; they even migrate into the genital ridge. In vitro, FSSCs exhibit characteristics
of embryonic stem cells, including extended self-renewal; expression of stem cell marker genes, such as Pou5f1 ( Oct4 ), Stat3 , and Akp2 ( Tnap ) and growth as multicellular aggregates. We report that fetal tissue contains somatic stem cells with greater potency than
previously thought, which might form a new source of stem cells useful in somatic nuclear transfer and cell therapy.
Abstract
Fetal tissue contains somatic stem cells with greater potency than previously thought, which might form a new source of stem
cells useful in somatic nuclear transfer and cell therapy |
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ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod.104.031229 |