Rapid identification of Salmonella enterica subsp. arizonae and S. enterica subsp. diarizonae by real-time polymerase chain reaction

Abstract Reptiles are popular as pets, leading to an increased risk of human infections due to uncommon Salmonella strains including the Arizona group (subspecies arizonae and diarizonae ). We present a real-time Arizona-specific polymerase chain reaction demonstrating 100% specificity and 99.6% sen...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2009-08, Vol.64 (4), p.452-454
Hauptverfasser:	Hopkins, Katie L, Peters, Tansy M, Lawson, Andy J, Owen, Robert J
Format:	Artikel
Sprache:	eng
Schlagworte:	5′ Nuclease real-time PCR Animals Arizona Bacteriology Biological and medical sciences DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Humans Infectious Disease Infectious diseases Internal Medicine Medical sciences Microbiology Miscellaneous Polymerase Chain Reaction - economics Polymerase Chain Reaction - methods Reptiles - microbiology Salmonella arizonae - classification Salmonella arizonae - isolation & purification Salmonella enterica Salmonella enterica - classification Salmonella enterica - isolation & purification Salmonella enterica subsp. arizonae Salmonella enterica subsp. diarizonae Salmonella Infections - diagnosis Salmonella Infections - microbiology Sensitivity and Specificity Time Factors
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Zusammenfassung:	Abstract Reptiles are popular as pets, leading to an increased risk of human infections due to uncommon Salmonella strains including the Arizona group (subspecies arizonae and diarizonae ). We present a real-time Arizona-specific polymerase chain reaction demonstrating 100% specificity and 99.6% sensitivity, offering savings in time and labor over traditional identification methods.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2009.03.022