High-efficiency protein extraction from polyacrylamide gels for molecular mass measurement by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

A simple and fast method of protein extraction from Coomassie Brilliant Blue (CBB)‐stained polyacrylamide gels suited for molecular mass measurement of proteins by matrix‐assisted laser desorption/ionization‐time of flight‐mass spectrometry (MALDI‐TOF‐MS) is reported. Proteins in CBB‐stained gel pieces were extracted by a 10‐min soaking in 0.1 M NaOH at 25°C. The recovery of this one‐step extraction method was 34–73% for proteins